Human HPRT1 ELISA Kit

Alternative name
Human HGPRT ELISA Kit;Human HGPRTase ELISA Kit;Human HPRT ELISA Kit;Human hypoxanthine phosphoribosyltransferase 1 ELISA Kit;Human hypoxanthine-guanine phosphoribosyltransferase ELISA Kit;Human hypoxanthine-guanine phosphoribosyltransferase 1 ELISA Kit;Human testicular tissue protein Li 89 ELISA Kit;
Catalog
E019181
species
Human
GeneHPRT1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). No significant cross-reactivity or interference between Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseHuman HPRT1 ELISA Kit allows for the in vitro quantitative determination of HPRT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxanthine Phosphoribosyltransferase 1 (HPRT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Hypoxanthine-guanine phosphoribosyltransferase Protein information
Uniprot ID HPRT_HUMAN
Uniprot AC P00492; A6NHF0; B2R8M9;
UniGene Hs.412707;
GeneID 3251
KEGG hsa:3251;
Human Hypoxanthine-guanine phosphoribosyltransferase Protein SEQUENCE
SEQUENCE 218 AA; 24579 MW; 1928EE69517CCB40 CRC64;
MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA
RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV
RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR
DLNHVCVISE TGKAKYKA
UCSC uc004exl.5; human.;

Human HPRT1 ELISA Kit

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