Human HSD17B4 ELISA Kit

Alternative name
Human MFE-2 ELISA Kit;Human 17-beta-hydroxysteroid dehydrogenase 4 ELISA Kit;Human 17-beta-HSD 4 ELISA Kit;Human D-bifunctional protein ELISA Kit;Human DBP ELISA Kit;Human multifunctional protein 2 ELISA Kit;Human MPF-2 ELISA Kit;Human short chain dehydrogenase/reductase family 8C member 1 ELISA Kit;Human 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase ELISA Kit;Human EDH17B4 ELISA Kit;Human SDR8C1 ELISA Kit;Human PRLTS1 ELISA Kit;Human hydroxysteroid 17-beta dehydrogenase 4 ELISA Kit;Human peroxisomal multifunctional enzyme type 2 ELISA Kit;Human 17-beta-HSD IV ELISA Kit;Human 17beta-estradiol dehydrogenase type IV ELISA Kit;Human D-3-hydroxyacyl-CoA dehydratase ELISA Kit;Human D-bifunctional protein, peroxisomal ELISA Kit;Human beta-hydroxyacyl dehydrogenase ELISA Kit;Human beta-keto-reductase ELISA Kit;Human hydroxysteroid dehydrogenase 4 ELISA Kit;Human peroxisomal multifunctional protein 2 ELISA Kit;
Catalog
E019164
species
Human
GeneHSD17B4
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HSD17B4. No significant cross-reactivity or interference between human HSD17B4 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HSD17B4 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseHuman HSD17B4 ELISA Kit allows for the in vitro quantitative determination of HSD17B4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSD17B4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSD17B4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSD17B4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSD17B4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Enoyl-CoA hydratase 2 Protein information
Uniprot ID DHB4_HUMAN
Uniprot AC P51659; B4DNV1; B4DVS5; E9PB82; F5HE57;
UniGene Hs.406861;
GeneID 3295
KEGG hsa:3295;
Human Enoyl-CoA hydratase 2 Protein SEQUENCE
SEQUENCE 736 AA; 79686 MW; 7B11E02483328BCE CRC64;
MGSPLRFDGR VVLVTGAGAG LGRAYALAFA ERGALVVVND LGGDFKGVGK
GSLAADKVVE EIRRRGGKAV ANYDSVEEGE KVVKTALDAF GRIDVVVNNA
GILRDRSFAR ISDEDWDIIH RVHLRGSFQV TRAAWEHMKK QKYGRIIMTS
SASGIYGNFG QANYSAAKLG LLGLANSLAI EGRKSNIHCN TIAPNAGSRM
TQTVMPEDLV EALKPEYVAP LVLWLCHESC EENGGLFEVG AGWIGKLRWE
RTLGAIVRQK NHPMTPEAVK ANWKKICDFE NASKPQSIQE STGSIIEVLS
KIDSEGGVSA NHTSRATSTA TSGFAGAIGQ KLPPFSYAYT ELEAIMYALG
VGASIKDPKD LKFIYEGSSD FSCLPTFGVI IGQKSMMGGG LAEIPGLSIN
FAKVLHGEQY LELYKPLPRA GKLKCEAVVA DVLDKGSGVV IIMDVYSYSE
KELICHNQFS LFLVGSGGFG GKRTSDKVKV AVAIPNRPPD AVLTDTTSLN
QAALYRLSGD WNPLHIDPNF ASLAGFDKPI LHGLCTFGFS ARRVLQQFAD
NDVSRFKAIK ARFAKPVYPG QTLQTEMWKE GNRIHFQTKV QETGDIVISN
AYVDLAPTSG TSAKTPSEGG KLQSTFVFEE IGRRLKDIGP EVVKKVNAVF
EWHITKGGNI GAKWTIDLKS GSGKVYQGPA KGAADTTIIL SDEDFMEVVL
GKLDPQKAFF SGRLKARGNI MLSQKLQMIL KDYAKL
UCSC uc003ksj.4; human. [P51659-1];

Category

Human HSD17B4 ELISA Kit