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Human HAGH ELISA Kit

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  • Alternative name

    Human glyoxalase II ELISA Kit;Human glx II ELISA Kit;Human GLO2 ELISA Kit;Human HAGH1 ELISA Kit;Human GLX2 ELISA Kit;Human GLXII ELISA Kit;Human hydroxyacylglutathione hydrolase ELISA Kit;Human hydroxyacylglutathione hydrolase, mitochondrial ELISA Kit;Human hydroxyacylglutathione hydroxylase ELISA Kit;
  • Catalog
    E019133
  • species
    Human
  • GeneHAGH
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HHM. No significant cross-reactivity or interference between HHM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HHM and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman HAGH ELISA Kit allows for the in vitro quantitative determination of HAGH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: HHM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HHM antibody and an HHM-HRP conjugate. The assay sample and buffer are incubated together with HHM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HHM concentration since HHM from samples and HHM-HRP conjugate compete for the anti-HHM antibody binding site. Since the number of sites is limited, as more sites are occupied by HHM from the sample, fewer sites are left to bind HHM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HHM concentration in each sample is interpolated from this standard curve.
  • Human Hydroxyacylglutathione hydrolase, mitochondrial Protein information
  • Uniprot ID GLO2_HUMAN
  • Uniprot AC Q16775; A8K290; B4DP33; B4DRA7; E7EN93;
  • UniGene Hs.157394;
  • GeneID 3029
  • KEGG hsa:3029;
  • Human Hydroxyacylglutathione hydrolase, mitochondrial Protein SEQUENCE

  • SEQUENCE 308 AA; 33806 MW; 64E5C214B6EC61CB CRC64;

    MVVGRGLLGR RSLAALGAAC ARRGLGPALL GVFCHTDLRK NLTVDEGTMK

    VEVLPALTDN YMYLVIDDET KEAAIVDPVQ PQKVVDAARK HGVKLTTVLT

    THHHWDHAGG NEKLVKLESG LKVYGGDDRI GALTHKITHL STLQVGSLNV

    KCLATPCHTS GHICYFVSKP GGSEPPAVFT GDTLFVAGCG KFYEGTADEM

    CKALLEVLGR LPPDTRVYCG HEYTINNLKF ARHVEPGNAA IREKLAWAKE

    KYSIGEPTVP STLAEEFTYN PFMRVREKTV QQHAGETDPV TTMRAVRREK

    DQFKMPRD

  • UCSC uc002cmz.4; human. [Q16775-1];



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