Human HYAL4 ELISA Kit

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  • Alternative name

    Human hyal-4 ELISA Kit;Human chondroitin sulfate endo-beta-N-acetylgalactosaminidase ELISA Kit;Human chondroitin sulfate hydrolase ELISA Kit;Human CSHY ELISA Kit;Human Hyaluronoglucosaminidase-4 ELISA Kit;Human hyaluronoglucosaminidase 4 ELISA Kit;Human hyaluronidase-4 ELISA Kit;

  • Catalog
    E019096
  • species
    Human
  • GeneHYAL4
  • Standard CurveHuman HYAL4 ELISA Kit
  • Other Species Mouse Hyal4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HYAL4 . No significant cross-reactivity or interference between HYAL4 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.188ng/ml
  • Intended UseHuman HYAL4 ELISA Kit allows for the in vitro quantitative determination of HYAL4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HYAL4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HYAL4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HYAL4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HYAL4 can be calculated.
  • Human Hyaluronidase-4 Protein information
  • Uniprot ID HYAL4_HUMAN
  • Uniprot AC Q2M3T9; D0VXG1; Q9UL99; Q9Y6T9;
  • UniGene Hs.28673;
  • GeneID 23553
  • KEGG hsa:23553;
  • Human Hyaluronidase-4 Protein SEQUENCE
  • SEQUENCE 481 AA; 54249 MW; 9D530009AA898D1F CRC64;

    MKVLSEGQLK LCVVQPVHLT SWLLIFFILK SISCLKPARL PIYQRKPFIA

    AWNAPTDQCL IKYNLRLNLK MFPVIGSPLA KARGQNVTIF YVNRLGYYPW

    YTSQGVPING GLPQNISLQV HLEKADQDIN YYIPAEDFSG LAVIDWEYWR

    PQWARNWNSK DVYRQKSRKL ISDMGKNVSA TDIEYLAKVT FEESAKAFMK

    ETIKLGIKSR PKGLWGYYLY PDCHNYNVYA PNYSGSCPED EVLRNNELSW

    LWNSSAALYP SIGVWKSLGD SENILRFSKF RVHESMRIST MTSHDYALPV

    FVYTRLGYRD EPLFFLSKQD LVSTIGESAA LGAAGIVIWG DMNLTASKAN

    CTKVKQFVSS DLGSYIANVT RAAEVCSLHL CRNNGRCIRK MWNAPSYLHL

    NPASYHIEAS EDGEFTVKGK ASDTDLAVMA DTFSCHCYQG YEGADCREIK

    TADGCSGVSP SPGSLMTLCL LLLASYRSIQ L

  • UCSC uc003vlc.4; human.;


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