Human HYAL2 ELISA Kit

Alternative name
Human hyal-2 ELISA Kit;Human Hyaluronoglucosaminidase-2 ELISA Kit;Human lung carcinoma protein 2 ELISA Kit;Human LuCa-2 ELISA Kit;Human LUCA2 ELISA Kit;Human hyaluronoglucosaminidase 2 ELISA Kit;Human hyaluronidase-2 ELISA Kit;Human PH-20 homolog ELISA Kit;Human PH20 homolog ELISA Kit;Human lysosomal hyaluronidase ELISA Kit;
Catalog
E019091
species
Human
GeneHYAL2
SpecificityThis assay has high sensitivity and excellent specificity for detection of HYAL2 . No significant cross-reactivity or interference between HYAL2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.188ng/ml
Intended UseHuman HYAL2 ELISA Kit allows for the in vitro quantitative determination of HYAL2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HYAL2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HYAL2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HYAL2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HYAL2 can be calculated.
Human Hyaluronidase-2 Protein information
Uniprot ID HYAL2_HUMAN
Uniprot AC Q12891; B3KRZ2; O15177; Q9BW29;
UniGene Hs.76873;
GeneID 8692
KEGG hsa:8692;
Human Hyaluronidase-2 Protein SEQUENCE
SEQUENCE 473 AA; 53860 MW; A8302738478BFE61 CRC64;
MRAGPGPTVT LALVLAVSWA MELKPTAPPI FTGRPFVVAW DVPTQDCGPR
LKVPLDLNAF DVQASPNEGF VNQNITIFYR DRLGLYPRFD SAGRSVHGGV
PQNVSLWAHR KMLQKRVEHY IRTQESAGLA VIDWEDWRPV WVRNWQDKDV
YRRLSRQLVA SRHPDWPPDR IVKQAQYEFE FAAQQFMLET LRYVKAVRPR
HLWGFYLFPD CYNHDYVQNW ESYTGRCPDV EVARNDQLAW LWAESTALFP
SVYLDETLAS SRHGRNFVSF RVQEALRVAR THHANHALPV YVFTRPTYSR
RLTGLSEMDL ISTIGESAAL GAAGVILWGD AGYTTSTETC QYLKDYLTRL
LVPYVVNVSW ATQYCSRAQC HGHGRCVRRN PSASTFLHLS TNSFRLVPGH
APGEPQLRPV GELSWADIDH LQTHFRCQCY LGWSGEQCQW DHRQAAGGAS
EAWAGSHLTS LLALAALAFT WTL
UCSC uc003czv.4; human.;

Category

Human HYAL2 ELISA Kit