Human HTRA4 ELISA Kit

Alternative name
Human high-temperature requirement factor A4 ELISA Kit;Human HtrA serine peptidase 4 ELISA Kit;Human serine protease HTRA4 ELISA Kit;Human probable serine protease HTRA4 ELISA Kit;
Catalog
E019026
species
Human
GeneHTRA4
Other Species Mouse Htra4 ELISA Kit Bovine HTRA4 ELISA Kit Rat Htra4 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of HTRA4. No significant cross-reactivity or interference between HTRA4 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.33ng/mL
Intended UseHuman HTRA4 ELISA Kit allows for the in vitro quantitative determination of HTRA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HTRA4 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to HTRA4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HTRA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HTRA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HTRA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Serine protease HTRA4 Protein information
Uniprot ID HTRA4_HUMAN
Uniprot AC P83105; Q542Z4; Q6PF13;
UniGene Hs.661014;
GeneID 203100
KEGG hsa:203100;
Human Serine protease HTRA4 Protein SEQUENCE
SEQUENCE 476 AA; 50979 MW; D458FFFAB4AF4328 CRC64;
MIRPQLRTAG LGRCLLPGLL LLLVPVLWAG AEKLHTQPSC PAVCQPTRCP
ALPTCALGTT PVFDLCRCCR VCPAAEREVC GGAQGQPCAP GLQCLQPLRP
GFPSTCGCPT LGGAVCGSDR RTYPSMCALR AENRAARRLG KVPAVPVQWG
NCGDTGTRSA GPLRRNYNFI AAVVEKVAPS VVHVQLWGRL LHGSRLVPVY
SGSGFIVSED GLIITNAHVV RNQQWIEVVL QNGARYEAVV KDIDLKLDLA
VIKIESNAEL PVLMLGRSSD LRAGEFVVAL GSPFSLQNTA TAGIVSTKQR
GGKELGMKDS DMDYVQIDAT INYGNSGGPL VNLDGDVIGV NSLRVTDGIS
FAIPSDRVRQ FLAEYHEHQM KGKAFSNKKY LGLQMLSLTV PLSEELKMHY
PDFPDVSSGV YVCKVVEGTA AQSSGLRDHD VIVNINGKPI TTTTDVVKAL
DSDSLSMAVL RGKDNLLLTV IPETIN
UCSC uc003xmj.4; human.;

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Human HTRA4 ELISA Kit

Human high-temperature requirement factor A4 ELISA Kit;Human HtrA serine peptidase 4 ELISA Kit;Human serine protease HTRA4 ELISA Kit;Human probable serine protease HTRA4 ELISA Kit;