Human HTRA4 ELISA Kit

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  • Alternative name

    Human high-temperature requirement factor A4 ELISA Kit;Human HtrA serine peptidase 4 ELISA Kit;Human serine protease HTRA4 ELISA Kit;Human probable serine protease HTRA4 ELISA Kit;

  • Catalog
    E019026
  • species
    Human
  • GeneHTRA4
  • Other Species Mouse Htra4 ELISA KitBovine HTRA4 ELISA KitRat Htra4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HTRA4. No significant cross-reactivity or interference between HTRA4 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.33ng/mL
  • Intended UseHuman HTRA4 ELISA Kit allows for the in vitro quantitative determination of HTRA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HTRA4 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to HTRA4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HTRA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HTRA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HTRA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Serine protease HTRA4 Protein information
  • Uniprot ID HTRA4_HUMAN
  • Uniprot AC P83105; Q542Z4; Q6PF13;
  • UniGene Hs.661014;
  • GeneID 203100
  • KEGG hsa:203100;
  • Human Serine protease HTRA4 Protein SEQUENCE
  • SEQUENCE 476 AA; 50979 MW; D458FFFAB4AF4328 CRC64;

    MIRPQLRTAG LGRCLLPGLL LLLVPVLWAG AEKLHTQPSC PAVCQPTRCP

    ALPTCALGTT PVFDLCRCCR VCPAAEREVC GGAQGQPCAP GLQCLQPLRP

    GFPSTCGCPT LGGAVCGSDR RTYPSMCALR AENRAARRLG KVPAVPVQWG

    NCGDTGTRSA GPLRRNYNFI AAVVEKVAPS VVHVQLWGRL LHGSRLVPVY

    SGSGFIVSED GLIITNAHVV RNQQWIEVVL QNGARYEAVV KDIDLKLDLA

    VIKIESNAEL PVLMLGRSSD LRAGEFVVAL GSPFSLQNTA TAGIVSTKQR

    GGKELGMKDS DMDYVQIDAT INYGNSGGPL VNLDGDVIGV NSLRVTDGIS

    FAIPSDRVRQ FLAEYHEHQM KGKAFSNKKY LGLQMLSLTV PLSEELKMHY

    PDFPDVSSGV YVCKVVEGTA AQSSGLRDHD VIVNINGKPI TTTTDVVKAL

    DSDSLSMAVL RGKDNLLLTV IPETIN

  • UCSC uc003xmj.4; human.;


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