Intended UseHuman HSV 1/2 ELISA Kit allows for the in vitro quantitative determination of HSV 1/2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyInfectious Disease; Immunology; Research Assays; Assay Categories
Product Description specificalIntended Use The Herpes 1/2 IgA Antibody ELISA Test Kit has been designed for the determination of specific IgA antibodies against Herpes 1/2 in serum and plasma. For research use only, not for use in diagnostic procedures.
Principle of the Test The Herpes 1/2 IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). Herpes 1/2 antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Herpes 1/2 antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm.