Human HSP-27/HSPB1 ELISA Kit

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  • Alternative name

    Heat shock protein beta-1 ELISA KIT; 28 kDa heat shock protein ELISA KIT; Estrogen-regulated 24 kDa protein ELISA KIT; Heat shock 27 kDa protein ELISA KIT; HSP 27 ELISA KIT; Stress-responsive protein 27 ELISA KIT; SRP27HSPB1 ELISA KIT; HSP27 ELISA KIT; HSP28 ELISA KIT; HspB1 ELISA KIT; HSP 27 ELISA KIT; SRP27 ELISA KIT

  • Catalog
    E018985
  • species
    Human
  • GeneHSP-27/HSPB1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human HSP-27/HSPB1. No significant cross-reactivity or interference between Human HSP-27/HSPB1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human HSP-27/HSPB1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.469ng/mL
  • Intended UseHuman HSP-27/HSPB1 ELISA Kit allows for the in vitro quantitative determination of HSP-27/HSPB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HSP-27/HSPB1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-27/HSPB1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human HSP-27/HSPB1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-27/HSPB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human HSP-27/HSPB1. You can calculate the concentration of Human HSP-27/HSPB1 in the samples by comparing the OD of the samples to the standard curve.


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