Human HLA-DRB1-15 ELISA Kit

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  • Alternative name

    HLA class II histocompatibility antigen, DRB1-15 beta chain ELISA KIT; DW2.2/DR2.2 ELISA KIT; MHC class II antigen DRB1*15HLA-DRB1 ELISA KIT; HLA-DRB2 ELISA KIT

  • Catalog
    E018865
  • species
    Human
  • GeneHLA-DRB1-15
  • Standard CurveHuman HLA-DRB1-15 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural Human HLA class II histocompatibility antigen, DRB1-15 beta chain. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman HLA-DRB1-15 ELISA Kit allows for the in vitro quantitative determination of HLA-DRB1-15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA class II histocompatibility antigen, DRB1-15 beta chain, During the reaction, HLA class II histocompatibility antigen, DRB1-15 beta chain in the sample or standard competes with a fixed amount of biotin-labeled HLA class II histocompatibility antigen, DRB1-15 beta chain for sites on a pre-coated Monoclonal antibody specific to HLA class II histocompatibility antigen, DRB1-15 beta chain. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HLA class II histocompatibility antigen, DRB1-15 beta chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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