Human H2B ELISA Kit

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  • Alternative name

    Histone H2B.10 ELISA KIT; HTB2At5g22880 ELISA KIT;

  • Catalog
    E018827
  • species
    Human
  • GeneH2B
  • Standard CurveHuman H2B ELISA Kit
  • Other Species Human H2A-H2B ELISA KitHuman Histon H2b ELISA KitMouse h2B ELISA KitMouse H2A-H2B ELISA KitMouse Histon-H2b ELISA KitBovine Histone H2B type 1 ELISA KitChicken H2B-VI ELISA KitRat Histone H2B type 1 ELISA KitChicken H2B-V ELISA KitChicken H2B-VII ELISA KitChicken H2B-VIII ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Histone H2B (H2B). No significant cross-reactivity or interference between Histone H2B (H2B) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.28ng/mL.
  • Intended UseHuman H2B ELISA Kit allows for the in vitro quantitative determination of H2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H2B (H2B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone H2B (H2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H2B (H2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone H2B (H2B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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