Intended UseHuman HHA ELISA Kit allows for the in vitro quantitative determination of HHA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyCell Biology
Product Description specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This HHA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HHA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY HHA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HHA antibody and an HHA-HRP conjugate. The assay sample and buffer are incubated together with HHA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HHA concentration since HHA from samples and HHA-HRP conjugate compete for the anti-HHA antibody binding site. Since the number of sites is limited, as more sites are occupied by HHA from the sample, fewer sites are left to bind HHA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HHA concentration in each sample is interpolated from this standard curve.