Human AMY2B ELISA Kit

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  • Alternative name

    Human 1,4-alpha-D-glucan glucanohydrolase 2B ELISA Kit;Human carcinoid alpha-amylase ELISA Kit;Human AMY2 ELISA Kit;Human AMY3 ELISA Kit;Human HXA ELISA Kit;Human amylase, alpha 2B (pancreatic) ELISA Kit;Human alpha-amylase 2B ELISA Kit;Human glycogenase ELISA Kit;

  • Catalog
    E001881
  • species
    Human
  • GeneAMY2B
  • Other Species Mouse AMY2B ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human AMY2B. No significant cross-reactivity or interference between human AMY2B and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human AMY2B is typically less than 9.77 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman AMY2B ELISA Kit allows for the in vitro quantitative determination of AMY2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMY2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMY2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMY2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMY2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Alpha-amylase 2B Protein information
  • Uniprot ID AMY2B_HUMAN
  • Uniprot AC P19961; B3KTI1; B3KXB7; D3DT76; Q9UBH3;
  • UniGene Hs.484588;
  • GeneID 280
  • KEGG hsa:280;
  • Human Alpha-amylase 2B Protein SEQUENCE
  • SEQUENCE 511 AA; 57710 MW; 05FC3B1EC1143857 CRC64;

    MKFFLLLFTI GFCWAQYSPN TQQGRTSIVH LFEWRWVDIA LECERYLAPK

    GFGGVQVSPP NENVAIHNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR

    CNNVGVRIYV DAVINHMSGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD

    FNDGKCKTGS GDIENYNDAT QVRDCRLVGL LDLALEKDYV RSKIAEYMNH

    LIDIGVAGFR LDASKHMWPG DIKAILDKLH NLNSNWFPAG SKPFIYQEVI

    DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG

    FMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF

    TRVMSSYRWP RQFQNGNDVN DWVGPPNNNG VIKEVTINPD TTCGNDWVCE

    HRWRQIRNMV NFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWT

    FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED

    PFIAIHAESK L

  • UCSC uc001duq.5; human. [P19961-1];



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