Human HDAC1 ELISA Kit

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  • Alternative name

    Human HD1 ELISA Kit;Human RPD3L1 ELISA Kit;Human GON-10 ELISA Kit;Human RPD3 ELISA Kit;Human histone deacetylase 1 ELISA Kit;Human reduced potassium dependency, yeast homolog-like 1 ELISA Kit;

  • Catalog
    E018791
  • species
    Human
  • GeneHDAC1
  • Standard CurveHuman HDAC1 ELISA Kit
  • Other Species Mouse Hdac1 ELISA KitBovine HDAC1 ELISA KitChicken HDAC1 ELISA KitRat Hdac1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Histone Deacetylase 1 (HDAC1). No significant cross-reactivity or interference between Histone Deacetylase 1 (HDAC1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman HDAC1 ELISA Kit allows for the in vitro quantitative determination of HDAC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 1 (HDAC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 1 (HDAC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Deacetylase 1 (HDAC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone Deacetylase 1 (HDAC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Histone deacetylase 1 Protein information
  • Uniprot ID HDAC1_HUMAN
  • Uniprot AC Q13547; Q92534;
  • UniGene Hs.88556;
  • GeneID 3065
  • KEGG hsa:3065;
  • Human Histone deacetylase 1 Protein SEQUENCE
  • SEQUENCE 482 AA; 55103 MW; 4D35B7C1ED7838D6 CRC64;

    MAQTQGTRRK VCYYYDGDVG NYYYGQGHPM KPHRIRMTHN LLLNYGLYRK

    MEIYRPHKAN AEEMTKYHSD DYIKFLRSIR PDNMSEYSKQ MQRFNVGEDC

    PVFDGLFEFC QLSTGGSVAS AVKLNKQQTD IAVNWAGGLH HAKKSEASGF

    CYVNDIVLAI LELLKYHQRV LYIDIDIHHG DGVEEAFYTT DRVMTVSFHK

    YGEYFPGTGD LRDIGAGKGK YYAVNYPLRD GIDDESYEAI FKPVMSKVME

    MFQPSAVVLQ CGSDSLSGDR LGCFNLTIKG HAKCVEFVKS FNLPMLMLGG

    GGYTIRNVAR CWTYETAVAL DTEIPNELPY NDYFEYFGPD FKLHISPSNM

    TNQNTNEYLE KIKQRLFENL RMLPHAPGVQ MQAIPEDAIP EESGDEDEDD

    PDKRISICSS DKRIACEEEF SDSEEEGEGG RKNSSNFKKA KRVKTEDEKE

    KDPEEKKEVT EEEKTKEEKP EAKGVKEEVK LA



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