SpecificityThis assay has high sensitivity and excellent specificity for detection of human HIST2H2BE. No significant cross-reactivity or interference between human HIST2H2BE and analogues was observed.
SensitivityThe minimum detectable dose of human HIST2H2BE is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined th
Intended UseHuman HIST2H2BE ELISA Kit allows for the in vitro quantitative determination of HIST2H2BE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIST2H2BE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIST2H2BE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIST2H2BE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIST2H2BE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Histone H2B type 2-E Protein information
Uniprot ID
H2B2E_HUMAN
Uniprot AC
Q16778; A3KMC7; A8K110; Q4KMY1; Q5QNX0; Q9UE88;