Human HIST1H4A ELISA Kit

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  • Alternative name

    Histone H4 ELISA KIT; HIST1H4A ELISA KIT; H4/A ELISA KIT; H4FA ELISA KIT

  • Catalog
    E018772
  • species
    Human
  • GeneHIST1H4A
  • Other Species Mouse Hist1h4a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human HIST1H4A. No significant cross-reactivity or interference between human HIST1H4A and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human HIST1H4A is typically less than 9.3 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
  • Intended UseHuman HIST1H4A ELISA Kit allows for the in vitro quantitative determination of HIST1H4A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIST1H4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIST1H4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIST1H4A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIST1H4A bound in the initial step. The color development is stopped and the intensity of the color is measured.



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