Human HAT1 ELISA Kit

Alternative name
Human histone acetyltransferase 1 ELISA Kit;Human KAT1 ELISA Kit;Human histone acetyltransferase type B catalytic subunit ELISA Kit;
Catalog
E018741
species
Human
GeneHAT1
Standard Curve
Other Species Mouse Hat1 ELISA Kit Rat Hat1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of HAT1. No significant cross-reactivity or interference between HAT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HAT1 is typically less than 0.103ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman HAT1 ELISA Kit allows for the in vitro quantitative determination of HAT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HAT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Histone acetyltransferase type B catalytic subunit Protein information
Uniprot ID HAT1_HUMAN
Uniprot AC O14929; Q49A44; Q53QF0; Q53SU4; Q6P594; Q8WWB9;
UniGene Hs.632532;
GeneID 8520
KEGG hsa:8520;
Human Histone acetyltransferase type B catalytic subunit Protein SEQUENCE
SEQUENCE 419 AA; 49513 MW; 509DF06E0E4E2647 CRC64;
MAGFGAMEKF LVEYKSAVEK KLAEYKCNTN TAIELKLVRF PEDLENDIRT
FFPEYTHQLF GDDETAFGYK GLKILLYYIA GSLSTMFRVE YASKVDENFD
CVEADDVEGK IRQIIPPGFC TNTNDFLSLL EKEVDFKPFG TLLHTYSVLS
PTGGENFTFQ IYKADMTCRG FREYHERLQT FLMWFIETAS FIDVDDERWH
YFLVFEKYNK DGATLFATVG YMTVYNYYVY PDKTRPRVSQ MLILTPFQGQ
GHGAQLLETV HRYYTEFPTV LDITAEDPSK SYVKLRDFVL VKLCQDLPCF
SREKLMQGFN EDMAIEAQQK FKINKQHARR VYEILRLLVT DMSDAEQYRS
YRLDIKRRLI SPYKKKQRDL AKMRKCLRPE ELTNQMNQIE ISMQHEQLEE
SFQELVEDYR RVIERLAQE
UCSC uc002uhi.4; human. [O14929-1];

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Human HAT1 ELISA Kit

Human histone acetyltransferase 1 ELISA Kit;Human KAT1 ELISA Kit;Human histone acetyltransferase type B catalytic subunit ELISA Kit;