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Alternative name
Histone H2A.Z ELISA KIT; H2AFZ ELISA KIT; H2AZ ELISA KIT; H2A/z ELISA KIT
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Catalog
E018740
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species
Human
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GeneHistone
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Standard Curve
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Other Species
Mouse Histone ELISA KitChicken Histone H1.01 ELISA KitChicken Histone H1.03 ELISA KitChicken Histone H1.10 ELISA KitChicken Histone H1.11L ELISA KitChicken Histone H1.11R ELISA KitRabbit Histone H1.3 ELISA KitChicken Histone H1.A2 ELISA KitChicken Histone H1 ELISA KitRat Histone H2A type 1-C ELISA KitRat Histone H2A type 1-E ELISA KitRat Histone H2A type 1-F ELISA KitBovine Histone H2A type 1 ELISA KitRat Histone H2A type 1 ELISA KitChicken Histone H2A-III ELISA KitRat Histone H2A type 3 ELISA KitChicken Histone H2A-IV ELISA KitRat Histone H2A type 4 ELISA KitBovine Histone H2B type 1 ELISA KitRat Histone H2B type 1 ELISA KitBovine Histone H3.1 ELISA KitRat Histone H3.1 ELISA KitBovine Histone H3.2 ELISA KitBovine Histone H3.3C-like ELISA KitBovine Histone H4 ELISA KitMonkey Histone H4 ELISA KitPorcine Histone H4 ELISA KitChicken Histone H5 ELISA KitChicken Non-histone chromosomal protein HMG-14A ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman Histone ELISA Kit allows for the in vitro quantitative determination of Histone , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCell Biology
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Product Description
specificalIntended Uses: This Histone ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Histone. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: Histone ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Histone antibody and an Histone-HRP conjugate. The assay sample and buffer are incubated together with Histone-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Histone concentration since Histone from samples and Histone-HRP conjugate compete for the anti-Histone antibody binding site. Since the number of sites is limited, as more sites are occupied by Histone from the sample, fewer sites are left to bind Histone-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Histone concentration in each sample is interpolated from this standard curve.
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