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Alternative name
AMY1 ELISA KIT; AMY1AMY1 ELISA KIT;
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Catalog
E001873
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species
Human
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GeneAMY1
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Standard Curve
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Other Species
Mouse Amy1 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of SAalpha1. No significant cross-reactivity or interference between SAalpha1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SAalpha1 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman AMY1 ELISA Kit allows for the in vitro quantitative determination of AMY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: SAalpha1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SAalpha1. Standards or samples are then added to the microtiter plate wells and SAalpha1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SAalpha1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SAalpha1 are added to each well to "sandwich" the SAalpha1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SAalpha1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAalpha1 concentration in each sample is interpolated from this standard curve.
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