Human HARS ELISA Kit

Alternative name
Human histidyl-tRNA synthetase ELISA Kit;Human HisRS ELISA Kit;Human HRS ELISA Kit;Human CMT2W ELISA Kit;Human USH3B ELISA Kit;Human histidine--tRNA ligase, cytoplasmic ELISA Kit;Human histidine translase ELISA Kit;
Catalog
E018724
species
Human
GeneHARS
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HARS. No significant cross-reactivity or interference between human HARS and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HARS is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman HARS ELISA Kit allows for the in vitro quantitative determination of HARS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HARS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HARS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Histidine--tRNA ligase, cytoplasmic Protein information
Uniprot ID SYHC_HUMAN
Uniprot AC P12081; B4DHQ1; B4DY73; D6REN6; J3KNE5;
UniGene Hs.528050;
GeneID 3035
KEGG hsa:3035;
Human Histidine--tRNA ligase, cytoplasmic Protein SEQUENCE
SEQUENCE 509 AA; 57411 MW; 65D8BB71CE79B1FF CRC64;
MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES
KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPVFELK
ETLMGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG NFDPMIPDAE CLKIMCEILS
SLQIGDFLVK VNDRRILDGM FAICGVSDSK FRTICSSVDK LDKVSWEEVK
NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG
DLKLLFEYLT LFGIDDKISF DLSLARGLDY YTGVIYEAVL LQTPAQAGEE
PLGVGSVAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL
EEKIRTTETQ VLVASAQKKL LEERLKLVSE LWDAGIKAEL LYKKNPKLLN
QLQYCEEAGI PLVAIIGEQE LKDGVIKLRS VTSREEVDVR REDLVEEIKR
RTGQPLCIC
UCSC uc003lgv.6; human. [P12081-1];

Category

Human HARS ELISA Kit

Human histidyl-tRNA synthetase ELISA Kit;Human HisRS ELISA Kit;Human HRS ELISA Kit;Human CMT2W ELISA Kit;Human USH3B ELISA Kit;Human histidine--tRNA ligase, cytoplasmic ELISA Kit;Human histidine translase ELISA Kit;