Human HARS ELISA Kit

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  • Alternative name

    Human histidyl-tRNA synthetase ELISA Kit;Human HisRS ELISA Kit;Human HRS ELISA Kit;Human CMT2W ELISA Kit;Human USH3B ELISA Kit;Human histidine--tRNA ligase, cytoplasmic ELISA Kit;Human histidine translase ELISA Kit;

  • Catalog
    E018724
  • species
    Human
  • GeneHARS
  • Other Species Mouse Hars ELISA KitBovine HARS ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human HARS. No significant cross-reactivity or interference between human HARS and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human HARS is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman HARS ELISA Kit allows for the in vitro quantitative determination of HARS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HARS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HARS bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Histidine--tRNA ligase, cytoplasmic Protein information
  • Uniprot ID SYHC_HUMAN
  • Uniprot AC P12081; B4DHQ1; B4DY73; D6REN6; J3KNE5;
  • UniGene Hs.528050;
  • GeneID 3035
  • KEGG hsa:3035;
  • Human Histidine--tRNA ligase, cytoplasmic Protein SEQUENCE
  • SEQUENCE 509 AA; 57411 MW; 65D8BB71CE79B1FF CRC64;

    MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES

    KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPVFELK

    ETLMGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY

    HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG NFDPMIPDAE CLKIMCEILS

    SLQIGDFLVK VNDRRILDGM FAICGVSDSK FRTICSSVDK LDKVSWEEVK

    NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG

    DLKLLFEYLT LFGIDDKISF DLSLARGLDY YTGVIYEAVL LQTPAQAGEE

    PLGVGSVAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL

    EEKIRTTETQ VLVASAQKKL LEERLKLVSE LWDAGIKAEL LYKKNPKLLN

    QLQYCEEAGI PLVAIIGEQE LKDGVIKLRS VTSREEVDVR REDLVEEIKR

    RTGQPLCIC

  • UCSC uc003lgv.6; human. [P12081-1];


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