Human HRC ELISA Kit

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  • Alternative name

    Human HCP ELISA Kit;Human histidine rich calcium binding protein ELISA Kit;Human sarcoplasmic reticulum histidine-rich calcium-binding protein ELISA Kit;

  • Catalog
    E018714
  • species
    Human
  • GeneHRC
  • Standard CurveHuman HRC ELISA Kit
  • Other Species Mouse HRC ELISA KitRabbit HRC ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Histidine Rich Calcium Binding Protein (HRC). No significant cross-reactivity or interference between Histidine Rich Calcium Binding Protein (HRC) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.123ng/mL.
  • Intended UseHuman HRC ELISA Kit allows for the in vitro quantitative determination of HRC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Rich Calcium Binding Protein (HRC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Rich Calcium Binding Protein (HRC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Rich Calcium Binding Protein (HRC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Rich Calcium Binding Protein (HRC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sarcoplasmic reticulum histidine-rich calcium-binding protein Protein information
  • Uniprot ID SRCH_HUMAN
  • Uniprot AC P23327; Q504Y6;
  • UniGene Hs.436885;
  • GeneID 3270
  • KEGG hsa:3270;
  • Human Sarcoplasmic reticulum histidine-rich calcium-binding protein Protein SEQUENCE
  • SEQUENCE 699 AA; 80244 MW; 9922EEDF012C61DD CRC64;

    MGHHRPWLHA SVLWAGVASL LLPPAMTQQL RGDGLGFRNR NNSTGVAGLS

    EEASAELRHH LHSPRDHPDE NKDVSTENGH HFWSHPDREK EDEDVSKEYG

    HLLPGHRSQD HKVGDEGVSG EEVFAEHGGQ ARGHRGHGSE DTEDSAEHRH

    HLPSHRSHSH QDEDEDEVVS SEHHHHILRH GHRGHDGEDD EGEEEEEEEE

    EEEEASTEYG HQAHRHRGHG SEEDEDVSDG HHHHGPSHRH QGHEEDDDDD

    DDDDDDDDDD DVSIEYRHQA HRHQGHGIEE DEDVSDGHHH RDPSHRHRSH

    EEDDNDDDDV STEYGHQAHR HQDHRKEEVE AVSGEHHHHV PDHRHQGHRD

    EEEDEDVSTE RWHQGPQHVH HGLVDEEEEE EEITVQFGHY VASHQPRGHK

    SDEEDFQDEY KTEVPHHHHH RVPREEDEEV SAELGHQAPS HRQSHQDEET

    GHGQRGSIKE MSHHPPGHTV VKDRSHLRKD DSEEEKEKEE DPGSHEEDDE

    SSEQGEKGTH HGSRDQEDEE DEEEGHGLSL NQEEEEEEDK EEEEEEEDEE

    RREERAEVGA PLSPDHSEEE EEEEEGLEED EPRFTIIPNP LDRREEAGGA

    SSEEESGEDT GPQDAQEYGN YQPGSLCGYC SFCNRCTECE SCHCDEENMG

    EHCDQCQHCQ FCYLCPLVCE TVCAPGSYVD YFSSSLYQAL ADMLETPEP

  • UCSC uc002pmv.4; human.;


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