Human HDC ELISA Kit

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  • Alternative name

    Human HDC ELISA Kit;Human histidine decarboxylase ELISA Kit;

  • Catalog
    E018711
  • species
    Human
  • GeneHDC
  • Other Species Mouse Hdc ELISA KitBovine HDC ELISA KitRat Hdc ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Histidine Decarboxylase (HDC). No significant cross-reactivity or interference between Histidine Decarboxylase (HDC) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.7ng/mL.
  • Intended UseHuman HDC ELISA Kit allows for the in vitro quantitative determination of HDC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Decarboxylase (HDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Decarboxylase (HDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Decarboxylase (HDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Decarboxylase (HDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Histidine decarboxylase Protein information
  • Uniprot ID DCHS_HUMAN
  • Uniprot AC P19113; A1L4G0; B7ZM01;
  • UniGene Hs.1481;
  • GeneID 3067
  • KEGG hsa:3067;
  • Human Histidine decarboxylase Protein SEQUENCE
  • SEQUENCE 662 AA; 74141 MW; D7611CFAAD60F469 CRC64;

    MMEPEEYRER GREMVDYICQ YLSTVRERRV TPDVQPGYLR AQLPESAPED

    PDSWDSIFGD IERIIMPGVV HWQSPHMHAY YPALTSWPSL LGDMLADAIN

    CLGFTWASSP ACTELEMNVM DWLAKMLGLP EHFLHHHPSS QGGGVLQSTV

    SESTLIALLA ARKNKILEMK TSEPDADESC LNARLVAYAS DQAHSSVEKA

    GLISLVKMKF LPVDDNFSLR GEALQKAIEE DKQRGLVPVF VCATLGTTGV

    CAFDCLSELG PICAREGLWL HIDAAYAGTA FLCPEFRGFL KGIEYADSFT

    FNPSKWMMVH FDCTGFWVKD KYKLQQTFSV NPIYLRHANS GVATDFMHWQ

    IPLSRRFRSV KLWFVIRSFG VKNLQAHVRH GTEMAKYFES LVRNDPSFEI

    PAKRHLGLVV FRLKGPNCLT ENVLKEIAKA GRLFLIPATI QDKLIIRFTV

    TSQFTTRDDI LRDWNLIRDA ATLILSQHCT SQPSPRVGNL ISQIRGARAW

    ACGTSLQSVS GAGDDPVQAR KIIKQPQRVG AGPMKRENGL HLETLLDPVD

    DCFSEEAPDA TKHKLSSFLF SYLSVQTKKK TVRSLSCNSV PVSAQKPLPT

    EASVKNGGSS RVRIFSRFPE DMMMLKKSAF KKLIKFYSVP SFPECSSQCG

    LQLPCCPLQA MV

  • UCSC uc001zxz.4; human. [P19113-1];



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