Human HAL ELISA Kit

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  • Alternative name

    Human histidase ELISA Kit;Human HIS ELISA Kit;Human HSTD ELISA Kit;Human histidine ammonia-lyase ELISA Kit;

  • Catalog
    E018707
  • species
    Human
  • GeneHAL
  • Standard CurveHuman HAL ELISA Kit
  • Other Species Bovine HAL ELISA KitMouse Hal ELISA KitRat Hal ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Histidine Ammonia Lyase (HAL). No significant cross-reactivity or interference between Histidine Ammonia Lyase (HAL) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.63ng/mL.
  • Intended UseHuman HAL ELISA Kit allows for the in vitro quantitative determination of HAL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Ammonia Lyase (HAL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Ammonia Lyase (HAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Ammonia Lyase (HAL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Ammonia Lyase (HAL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Histidine ammonia-lyase Protein information
  • Uniprot ID HUTH_HUMAN
  • Uniprot AC P42357; B4DQC1; B4E0V8; F5GXF2; F5H1U5; Q4VB92; Q4VB93;
  • UniGene Hs.190783; Hs.742210;
  • GeneID 3034
  • KEGG hsa:3034;
  • Human Histidine ammonia-lyase Protein SEQUENCE
  • SEQUENCE 657 AA; 72698 MW; 6B9FF97C066FB8F1 CRC64;

    MPRYTVHVRG EWLAVPCQDA QLTVGWLGRE AVRRYIKNKP DNGGFTSVDD

    AHFLVRRCKG LGLLDNEDRL EVALENNEFV EVVIEGDAMS PDFIPSQPEG

    VYLYSKYREP EKYIELDGDR LTTEDLVNLG KGRYKIKLTP TAEKRVQKSR

    EVIDSIIKEK TVVYGITTGF GKFARTVIPI NKLQELQVNL VRSHSSGVGK

    PLSPERCRML LALRINVLAK GYSGISLETL KQVIEMFNAS CLPYVPEKGT

    VGASGDLAPL SHLALGLVGE GKMWSPKSGW ADAKYVLEAH GLKPVILKPK

    EGLALINGTQ MITSLGCEAV ERASAIARQA DIVAALTLEV LKGTTKAFDT

    DIHALRPHRG QIEVAFRFRS LLDSDHHPSE IAESHRFCDR VQDAYTLRCC

    PQVHGVVNDT IAFVKNIITT ELNSATDNPM VFANRGETVS GGNFHGEYPA

    KALDYLAIGI HELAAISERR IERLCNPSLS ELPAFLVAEG GLNSGFMIAH

    CTAAALVSEN KALCHPSSVD SLSTSAATED HVSMGGWAAR KALRVIEHVE

    QVLAIELLAA CQGIEFLRPL KTTTPLEKVY DLVRSVVRPW IKDRFMAPDI

    EAAHRLLLEQ KVWEVAAPYI EKYRMEHIPE SRPLSPTAFS LQFLHKKSTK

    IPESEDL

  • UCSC uc001tem.2; human. [P42357-1];


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