Human HAL ELISA Kit

Alternative name
Human histidase ELISA Kit;Human HIS ELISA Kit;Human HSTD ELISA Kit;Human histidine ammonia-lyase ELISA Kit;
Catalog
E018707
species
Human
GeneHAL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Histidine Ammonia Lyase (HAL). No significant cross-reactivity or interference between Histidine Ammonia Lyase (HAL) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.63ng/mL.
Intended UseHuman HAL ELISA Kit allows for the in vitro quantitative determination of HAL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Ammonia Lyase (HAL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Ammonia Lyase (HAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Ammonia Lyase (HAL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Ammonia Lyase (HAL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Histidine ammonia-lyase Protein information
Uniprot ID HUTH_HUMAN
Uniprot AC P42357; B4DQC1; B4E0V8; F5GXF2; F5H1U5; Q4VB92; Q4VB93;
UniGene Hs.190783; Hs.742210;
GeneID 3034
KEGG hsa:3034;
Human Histidine ammonia-lyase Protein SEQUENCE
SEQUENCE 657 AA; 72698 MW; 6B9FF97C066FB8F1 CRC64;
MPRYTVHVRG EWLAVPCQDA QLTVGWLGRE AVRRYIKNKP DNGGFTSVDD
AHFLVRRCKG LGLLDNEDRL EVALENNEFV EVVIEGDAMS PDFIPSQPEG
VYLYSKYREP EKYIELDGDR LTTEDLVNLG KGRYKIKLTP TAEKRVQKSR
EVIDSIIKEK TVVYGITTGF GKFARTVIPI NKLQELQVNL VRSHSSGVGK
PLSPERCRML LALRINVLAK GYSGISLETL KQVIEMFNAS CLPYVPEKGT
VGASGDLAPL SHLALGLVGE GKMWSPKSGW ADAKYVLEAH GLKPVILKPK
EGLALINGTQ MITSLGCEAV ERASAIARQA DIVAALTLEV LKGTTKAFDT
DIHALRPHRG QIEVAFRFRS LLDSDHHPSE IAESHRFCDR VQDAYTLRCC
PQVHGVVNDT IAFVKNIITT ELNSATDNPM VFANRGETVS GGNFHGEYPA
KALDYLAIGI HELAAISERR IERLCNPSLS ELPAFLVAEG GLNSGFMIAH
CTAAALVSEN KALCHPSSVD SLSTSAATED HVSMGGWAAR KALRVIEHVE
QVLAIELLAA CQGIEFLRPL KTTTPLEKVY DLVRSVVRPW IKDRFMAPDI
EAAHRLLLEQ KVWEVAAPYI EKYRMEHIPE SRPLSPTAFS LQFLHKKSTK
IPESEDL
UCSC uc001tem.2; human. [P42357-1];

Category

Human HAL ELISA Kit

Human histidase ELISA Kit;Human HIS ELISA Kit;Human HSTD ELISA Kit;Human histidine ammonia-lyase ELISA Kit;