Human HRD ELISA Kit

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  • Catalog
    E018675
  • species
    Human
  • GeneHRD
  • Standard CurveHuman HRD ELISA Kit
  • Other Species Mouse HRD ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman HRD ELISA Kit allows for the in vitro quantitative determination of HRD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This HRD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HRD. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY HRD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HRD antibody and an HRD-HRP conjugate. The assay sample and buffer are incubated together with HRD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HRD concentration since HRD from samples and HRD-HRP conjugate compete for the anti-HRD antibody binding site. Since the number of sites is limited, as more sites are occupied by HRD from the sample, fewer sites are left to bind HRD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HRD concentration in each sample is interpolated from this standard curve.



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