Human HMGB4 ELISA Kit

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  • Alternative name

    Human dJ1007G16.5 ELISA Kit;Human high mobility group box 4 ELISA Kit;Human high mobility group protein B4 ELISA Kit;Human HMG2 like ELISA Kit;

  • Catalog
    E018635
  • species
    Human
  • GeneHMGB4
  • Other Species Mouse Hmgb4 ELISA KitBovine HMGB4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman HMGB4 ELISA Kit allows for the in vitro quantitative determination of HMGB4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HMGB4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HMGB4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HMGB4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HMGB4 can be calculated.
  • Human High mobility group protein B4 Protein information
  • Uniprot ID HMGB4_HUMAN
  • Uniprot AC Q8WW32; B2R4X7; Q0QWA4;
  • UniGene Hs.568628; Hs.667683;
  • GeneID 127540
  • KEGG hsa:127540;
  • Human High mobility group protein B4 Protein SEQUENCE
  • SEQUENCE 186 AA; 22490 MW; E7F941ABBC8D3025 CRC64;

    MGKEIQLKPK ANVSSYVHFL LNYRNKFKEQ QPNTYVGFKE FSRKCSEKWR

    SISKHEKAKY EALAKLDKAR YQEEMMNYVG KRKKRRKRDP QEPRRPPSSF

    LLFCQDHYAQ LKRENPNWSV VQVAKATGKM WSTATDLEKH PYEQRVALLR

    AKYFEELELY RKQCNARKKY RMSARNRCRG KRVRQS

  • UCSC uc001bxp.3; human.;


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