Human HMGB3 ELISA Kit

Alternative name
Human high mobility group protein 2a ELISA Kit;Human HMG-2a ELISA Kit;Human High mobility group protein 4 ELISA Kit;Human HMG-4 ELISA Kit;Human HMG2A ELISA Kit;Human HMG4 ELISA Kit;Human high mobility group box 3 ELISA Kit;Human high mobility group protein B3 ELISA Kit;Human high-mobility group (nonhistone chromosomal) protein 4 ELISA Kit;
Catalog
E018632
species
Human
GeneHMGB3
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman HMGB3 ELISA Kit allows for the in vitro quantitative determination of HMGB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HMGB3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HMGB3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HMGB3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HMGB3 can be calculated.
Human High mobility group protein B3 Protein information
Uniprot ID HMGB3_HUMAN
Uniprot AC O15347; O95556; Q6NS40;
UniGene Hs.19114;
GeneID 3149
KEGG hsa:3149;
Human High mobility group protein B3 Protein SEQUENCE
SEQUENCE 200 AA; 22980 MW; 31C090FAA4D581E6 CRC64;
MAKGDPKKPK GKMSAYAFFV QTCREEHKKK NPEVPVNFAE FSKKCSERWK
TMSGKEKSKF DEMAKADKVR YDREMKDYGP AKGGKKKKDP NAPKRPPSGF
FLFCSEFRPK IKSTNPGISI GDVAKKLGEM WNNLNDSEKQ PYITKAAKLK
EKYEKDVADY KSKGKFDGAK GPAKVARKKV EEEDEEEEEE EEEEEEEEDE
UCSC uc004fep.4; human.;

Human HMGB3 ELISA Kit

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