Human HMGB1 ELISA Kit

Alternative name
Human High mobility group protein 1 ELISA Kit;Human HMG-1 ELISA Kit;Human HMG1 ELISA Kit;Human HMG3 ELISA Kit;Human SBP-1 ELISA Kit;Human high mobility group box 1 ELISA Kit;Human high mobility group protein B1 ELISA Kit;Human Amphoterin ELISA Kit;Human Sulfoglucuronyl carbohydrate binding protein ELISA Kit;Human high-mobility group (nonhistone chromosomal) protein 1 ELISA Kit;
Catalog
E018616
species
Human
GeneHMGB1
SpecificityThis assay has high sensitivity and excellent specificity for detection of HMGB1. No significant cross-reactivity or interference between HMGB1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HMGB1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman HMGB1 ELISA Kit allows for the in vitro quantitative determination of HMGB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPrinciple of the assay: HMGB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HMGB1 antibody and an HMGB1-HRP conjugate. The assay sample and buffer are incubated together with HMGB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMGB1 concentration since HMGB1 from samples and HMGB1-HRP conjugate compete for the anti-HMGB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HMGB1 from the sample, fewer sites are left to bind HMGB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGB1 concentration in each sample is interpolated from this standard curve.
Human High mobility group protein B1 Protein information
Uniprot ID HMGB1_HUMAN
Uniprot AC P09429; A5D8W9; Q14321; Q5T7C3; Q6IBE1;
UniGene Hs.434102; Hs.593339; Hs.596078;
GeneID 3146
KEGG hsa:3146;
Human High mobility group protein B1 Protein SEQUENCE
SEQUENCE 215 AA; 24894 MW; 8A868CF277D417B5 CRC64;
MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK
TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS
AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK
LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE
EDEEDEDEEE DDDDE
UCSC uc001usx.5; human.;

Human HMGB1 ELISA Kit

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