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Human HMGA1 ELISA Kit

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  • Alternative name

    Human HMG-I(Y) ELISA Kit;Human High mobility group AT-hook protein 1 ELISA Kit;Human high mobility group protein A1 ELISA Kit;Human high mobility group protein R ELISA Kit;Human HMGIY ELISA Kit;Human HMG-R ELISA Kit;Human HMGA1A ELISA Kit;Human high mobility group AT-hook 1 ELISA Kit;Human high mobility group protein HMG-I/HMG-Y ELISA Kit;Human high-mobility group (nonhistone chromosomal) protein isoforms I and Y ELISA Kit;Human nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y ELISA Kit;
  • Catalog
    E018612
  • species
    Human
  • GeneHMGA1
  • Standard CurveHuman HMGA1 ELISA Kit
  • Other Species Mouse Hmga1 ELISA KitCanine HMGA1 ELISA KitHamster HMGA1 ELISA KitRat Hmga1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HMGA1. No significant cross-reactivity or interference between HMGA1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human HMGA1 is typically less than 0.124ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
  • Intended UseHuman HMGA1 ELISA Kit allows for the in vitro quantitative determination of HMGA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMGA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HMGA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HMGA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HMGA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human High mobility group protein HMG-I/HMG-Y Protein information
  • Uniprot ID HMGA1_HUMAN
  • Uniprot AC P17096; P10910; Q5T6U9; Q9UKB0;
  • UniGene Hs.518805; Hs.696527; Hs.703764;
  • GeneID 3159
  • KEGG hsa:3159;
  • Human High mobility group protein HMG-I/HMG-Y Protein SEQUENCE

  • SEQUENCE 107 AA; 11676 MW; E9C4E3F2200914B8 CRC64;

    MSESSSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE

    VPTPKRPRGR PKGSKNKGAA KTRKTTTTPG RKPRGRPKKL EKEEEEGISQ

    ESSEEEQ

  • UCSC uc003oit.4; human. [P17096-1];



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