Human HMGA1 ELISA Kit

Alternative name
Human HMG-I(Y) ELISA Kit;Human High mobility group AT-hook protein 1 ELISA Kit;Human high mobility group protein A1 ELISA Kit;Human high mobility group protein R ELISA Kit;Human HMGIY ELISA Kit;Human HMG-R ELISA Kit;Human HMGA1A ELISA Kit;Human high mobility group AT-hook 1 ELISA Kit;Human high mobility group protein HMG-I/HMG-Y ELISA Kit;Human high-mobility group (nonhistone chromosomal) protein isoforms I and Y ELISA Kit;Human nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y ELISA Kit;
Catalog
E018612
species
Human
GeneHMGA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of HMGA1. No significant cross-reactivity or interference between HMGA1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HMGA1 is typically less than 0.124ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
Intended UseHuman HMGA1 ELISA Kit allows for the in vitro quantitative determination of HMGA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMGA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HMGA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HMGA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HMGA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human High mobility group protein HMG-I/HMG-Y Protein information
Uniprot ID HMGA1_HUMAN
Uniprot AC P17096; P10910; Q5T6U9; Q9UKB0;
UniGene Hs.518805; Hs.696527; Hs.703764;
GeneID 3159
KEGG hsa:3159;
Human High mobility group protein HMG-I/HMG-Y Protein SEQUENCE
SEQUENCE 107 AA; 11676 MW; E9C4E3F2200914B8 CRC64;
MSESSSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE
VPTPKRPRGR PKGSKNKGAA KTRKTTTTPG RKPRGRPKKL EKEEEEGISQ
ESSEEEQ
UCSC uc003oit.4; human. [P17096-1];

Human HMGA1 ELISA Kit

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