Human HDLBP ELISA Kit

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  • Alternative name

    Human High density lipoprotein-binding protein ELISA Kit;Human HDL-binding protein ELISA Kit;Human HBP ELISA Kit;Human VGL ELISA Kit;Human PRO2900 ELISA Kit;Human high density lipoprotein binding protein ELISA Kit;Human vigilin ELISA Kit;

  • Catalog
    E018602
  • species
    Human
  • GeneHDLBP
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of High Density Lipoprotein Binding Protein (HDLBP). No significant cross-reactivity or interference between High Density Lipoprotein Binding Protein (HDLBP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.056ng/mL.
  • Intended UseHuman HDLBP ELISA Kit allows for the in vitro quantitative determination of HDLBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Density Lipoprotein Binding Protein (HDLBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Density Lipoprotein Binding Protein (HDLBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Vigilin Protein information
  • Uniprot ID VIGLN_HUMAN
  • Uniprot AC Q00341; B4DTQ2; E7EM71; Q53QU2; Q9UCY3;
  • UniGene Hs.471851; Hs.732361;
  • GeneID 3069
  • KEGG hsa:3069;
  • Human Vigilin Protein SEQUENCE
  • SEQUENCE 1268 AA; 141456 MW; 530C61A3CA9239CD CRC64;

    MSSVAVLTQE SFAEHRSGLV PQQIKVATLN SEEESDPPTY KDAFPPLPEK

    AACLESAQEP SGAWGNKIRP IKASVITQVF HVPLEERKYK DMNQFGEGEQ

    AKICLEIMQR TGAHLELSLA KDQGLSIMVS GKLDAVMKAR KDIVARLQTQ

    ASATVAIPKE HHRFVIGKNG EKLQDLELKT ATKIQIPRPD DPSNQIKITG

    TKEGIEKARH EVLLISAEQD KRAVERLEVE KAFHPFIAGP YNRLVGEIMQ

    ETGTRINIPP PSVNRTEIVF TGEKEQLAQA VARIKKIYEE KKKKTTTIAV

    EVKKSQHKYV IGPKGNSLQE ILERTGVSVE IPPSDSISET VILRGEPEKL

    GQALTEVYAK ANSFTVSSVA APSWLHRFII GKKGQNLAKI TQQMPKVHIE

    FTEGEDKITL EGPTEDVNVA QEQIEGMVKD LINRMDYVEI NIDHKFHRHL

    IGKSGANINR IKDQYKVSVR IPPDSEKSNL IRIEGDPQGV QQAKRELLEL

    ASRMENERTK DLIIEQRFHR TIIGQKGERI REIRDKFPEV IINFPDPAQK

    SDIVQLRGPK NEVEKCTKYM QKMVADLVEN SYSISVPIFK QFHKNIIGKG

    GANIKKIREE SNTKIDLPAE NSNSETIIIT GKRANCEAAR SRILSIQKDL

    ANIAEVEVSI PAKLHNSLIG TKGRLIRSIM EECGGVHIHF PVEGSGSDTV

    VIRGPSSDVE KAKKQLLHLA EEKQTKSFTV DIRAKPEYHK FLIGKGGGKI

    RKVRDSTGAR VIFPAAEDKD QDLITIIGKE DAVREAQKEL EALIQNLDNV

    VEDSMLVDPK HHRHFVIRRG QVLREIAEEY GGVMVSFPRS GTQSDKVTLK

    GAKDCVEAAK KRIQEIIEDL EAQVTLECAI PQKFHRSVMG PKGSRIQQIT

    RDFSVQIKFP DREENAVHST EPVVQENGDE AGEGREAKDC DPGSPRRCDI

    IIISGRKEKC EAAKEALEAL VPVTIEVEVP FDLHRYVIGQ KGSGIRKMMD

    EFEVNIHVPA PELQSDIIAI TGLAANLDRA KAGLLERVKE LQAEQEDRAL

    RSFKLSVTVD PKYHPKIIGR KGAVITQIRL EHDVNIQFPD KDDGNQPQDQ

    ITITGYEKNT EAARDAILRI VGELEQMVSE DVPLDHRVHA RIIGARGKAI

    RKIMDEFKVD IRFPQSGAPD PNCVTVTGLP ENVEEAIDHI LNLEEEYLAD

    VVDSEALQVY MKPPAHEEAK APSRGFVVRD APWTASSSEK APDMSSSEEF

    PSFGAQVAPK TLPWGPKR

  • UCSC uc021vzg.1; human. [Q00341-1];



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