Human AREG ELISA Kit

Alternative name
Human AR ELISA Kit;Human colorectum cell-derived growth factor ELISA Kit;Human CRDGF ELISA Kit;Human AREGB ELISA Kit;Human SDGF ELISA Kit;Human amphiregulin ELISA Kit;Human amphiregulin B ELISA Kit;Human schwannoma-derived growth factor ELISA Kit;
Catalog
E001858
species
Human
GeneAREG
Standard Curve
Other Species Mouse Areg ELISA Kit Rat Areg ELISA Kit
SpecificityThe Human Amphiregulin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Amphiregulin proteins within the range of 15.6 pg/ml - 1000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of Human Amphiregulin is typically less than 10 pg/ml.
Intended UseHuman AREG ELISA Kit allows for the in vitro quantitative determination of AREG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The Human Amphiregulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amphiregulin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background: Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.
Human Amphiregulin Protein information
Uniprot ID AREG_HUMAN
Uniprot AC P15514; Q5U026;
UniGene Hs.270833; Hs.645475;
GeneID 374
KEGG hsa:374;
Human Amphiregulin Protein SEQUENCE
SEQUENCE 252 AA; 27895 MW; 68C1FA6BCD4D2BED CRC64;
MRAPLLPPAP VVLSLLILGS GHYAAGLDLN DTYSGKREPF SGDHSADGFE
VTSRSEMSSG SEISPVSEMP SSSEPSSGAD YDYSEEYDNE PQIPGYIVDD
SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF
QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSKIA
LAAIAAFMSA VILTAVAVIT VQLRRQYVRK YEGEAEERKK LRQENGNVHA
IA
UCSC uc032tme.2; human.;

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Category

Human AREG ELISA Kit

Human AR ELISA Kit;Human colorectum cell-derived growth factor ELISA Kit;Human CRDGF ELISA Kit;Human AREGB ELISA Kit;Human SDGF ELISA Kit;Human amphiregulin ELISA Kit;Human amphiregulin B ELISA Kit;Human schwannoma-derived growth factor ELISA Kit;