Human HEXA ELISA Kit

Alternative name
Human beta-N-acetylhexosaminidase subunit alpha ELISA Kit;Human hexosaminidase subunit A ELISA Kit;Human N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit;Human TSD ELISA Kit;Human hexosaminidase subunit alpha ELISA Kit;Human beta-hexosaminidase subunit alpha ELISA Kit;Human hexosaminidase A (alpha polypeptide) ELISA Kit;
Catalog
E018558
species
Human
GeneHEXA
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HEXA. No significant cross-reactivity or interference between human HEXA and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HEXA is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman HEXA ELISA Kit allows for the in vitro quantitative determination of HEXA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HEXA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HEXA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HEXA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HEXA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Beta-hexosaminidase subunit alpha Protein information
Uniprot ID HEXA_HUMAN
Uniprot AC P06865; B4DKE7; E7ENH7; Q53HS8; Q6AI32;
UniGene Hs.604479; Hs.709495;
GeneID 3073
KEGG hsa:3073;
Human Beta-hexosaminidase subunit alpha Protein SEQUENCE
SEQUENCE 529 AA; 60703 MW; DACB3E3992E57A47 CRC64;
MTSSRLWFSL LLAAAFAGRA TALWPWPQNF QTSDQRYVLY PNNFQFQYDV
SSAAQPGCSV LDEAFQRYRD LLFGSGSWPR PYLTGKRHTL EKNVLVVSVV
TPGCNQLPTL ESVENYTLTI NDDQCLLLSE TVWGALRGLE TFSQLVWKSA
EGTFFINKTE IEDFPRFPHR GLLLDTSRHY LPLSSILDTL DVMAYNKLNV
FHWHLVDDPS FPYESFTFPE LMRKGSYNPV THIYTAQDVK EVIEYARLRG
IRVLAEFDTP GHTLSWGPGI PGLLTPCYSG SEPSGTFGPV NPSLNNTYEF
MSTFFLEVSS VFPDFYLHLG GDEVDFTCWK SNPEIQDFMR KKGFGEDFKQ
LESFYIQTLL DIVSSYGKGY VVWQEVFDNK VKIQPDTIIQ VWREDIPVNY
MKELELVTKA GFRALLSAPW YLNRISYGPD WKDFYIVEPL AFEGTPEQKA
LVIGGEACMW GEYVDNTNLV PRLWPRAGAV AERLWSNKLT SDLTFAYERL
SHFRCELLRR GVQAQPLNVG FCEQEFEQT
UCSC uc002aun.5; human. [P06865-1];

Category

Human HEXA ELISA Kit