Human HK3 ELISA Kit

Alternative name
Human hexokinase type III ELISA Kit;Human HK III ELISA Kit;Human HKIII ELISA Kit;Human HXK3 ELISA Kit;Human hexokinase 3 ELISA Kit;Human hexokinase-3 ELISA Kit;Human ATP:D-hexose 6-phosphotransferase ELISA Kit;Human hexokinase 3 (white cell) ELISA Kit;
Catalog
E018551
species
Human
GeneHK3
Standard Curve
Other Species Mouse Hk3 ELISA Kit Rat Hk3 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman HK3 ELISA Kit allows for the in vitro quantitative determination of HK3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This HK3 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHK3 in the sample, thisHK3 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHK3 concentration. The concentration ofHK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This HK3 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHK3. Standards or samples are then added to the microtiter plate wells andHK3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHK3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHK3 are added to each well to "sandwich" theHK3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that containHK3 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Human Hexokinase-3 Protein information
Uniprot ID HXK3_HUMAN
Uniprot AC P52790; Q8N1E7;
UniGene Hs.411695;
GeneID 3101
KEGG hsa:3101;
Human Hexokinase-3 Protein SEQUENCE
SEQUENCE 923 AA; 99025 MW; DA75F2A9DBB895FF CRC64;
MDSIGSSGLR QGEETLSCSE EGLPGPSDSS ELVQECLQQF KVTRAQLQQI
QASLLGSMEQ ALRGQASPAP AVRMLPTYVG STPHGTEQGD FVVLELGATG
ASLRVLWVTL TGIEGHRVEP RSQEFVIPQE VMLGAGQQLF DFAAHCLSEF
LDAQPVNKQG LQLGFSFSFP CHQTGLDRST LISWTKGFRC SGVEGQDVVQ
LLRDAIRRQG AYNIDVVAVV NDTVGTMMGC EPGVRPCEVG LVVDTGTNAC
YMEEARHVAV LDEDRGRVCV SVEWGSFSDD GALGPVLTTF DHTLDHESLN
PGAQRFEKMI GGLYLGELVR LVLAHLARCG VLFGGCTSPA LLSQGSILLE
HVAEMEDPST GAARVHAILQ DLGLSPGASD VELVQHVCAA VCTRAAQLCA
AALAAVLSCL QHSREQQTLQ VAVATGGRVC ERHPRFCSVL QGTVMLLAPE
CDVSLIPSVD GGGRGVAMVT AVAARLAAHR RLLEETLAPF RLNHDQLAAV
QAQMRKAMAK GLRGEASSLR MLPTFVRATP DGSERGDFLA LDLGGTNFRV
LLVRVTTGVQ ITSEIYSIPE TVAQGSGQQL FDHIVDCIVD FQQKQGLSGQ
SLPLGFTFSF PCRQLGLDQG ILLNWTKGFK ASDCEGQDVV SLLREAITRR
QAVELNVVAI VNDTVGTMMS CGYEDPRCEI GLIVGTGTNA CYMEELRNVA
GVPGDSGRMC INMEWGAFGD DGSLAMLSTR FDASVDQASI NPGKQRFEKM
ISGMYLGEIV RHILLHLTSL GVLFRGQQIQ RLQTRDIFKT KFLSEIESDS
LALRQVRAIL EDLGLPLTSD DALMVLEVCQ AVSQRAAQLC GAGVAAVVEK
IRENRGLEEL AVSVGVDGTL YKLHPRFSSL VAATVRELAP RCVVTFLQSE
DGSGKGAALV TAVACRLAQL TRV
UCSC uc003mfa.4; human.;

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anti-HK3 antibody

Category

Human HK3 ELISA Kit

Human hexokinase type III ELISA Kit;Human HK III ELISA Kit;Human HKIII ELISA Kit;Human HXK3 ELISA Kit;Human hexokinase 3 ELISA Kit;Human hexokinase-3 ELISA Kit;Human ATP:D-hexose 6-phosphotransferase ELISA Kit;Human hexokinase 3 (white cell) ELISA Kit;