Human HK2 ELISA Kit

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  • Alternative name

    Human hexokinase type II ELISA Kit;Human HK II ELISA Kit;Human muscle form hexokinase ELISA Kit;Human HKII ELISA Kit;Human HXK2 ELISA Kit;Human hexokinase 2 ELISA Kit;Human hexokinase-2 ELISA Kit;Human hexokinase-2, muscle ELISA Kit;

  • Catalog
    E018548
  • species
    Human
  • GeneHK2
  • Standard CurveHuman HK2 ELISA Kit
  • Other Species Mouse Hk2 ELISA KitHorse HK2 ELISA KitPorcine HK2 ELISA KitRat Hk2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HK2. No significant cross-reactivity or interference between HK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HK2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL
  • Intended UseHuman HK2 ELISA Kit allows for the in vitro quantitative determination of HK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyHuman ELISA Kit
  • Product Description
    specifical
    Intended Uses: This HK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HK2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: HK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HK2 antibody and an HK2-HRP conjugate. The assay sample and buffer are incubated together with HK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HK2 concentration since HK2 from samples and HK2-HRP conjugate compete for the anti-HK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HK2 from the sample, fewer sites are left to bind HK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HK2 concentration in each sample is interpolated from this standard curve.
  • Human Hexokinase-2 Protein information
  • Uniprot ID HXK2_HUMAN
  • Uniprot AC P52789; D6W5J2; Q8WU87; Q9UN82;
  • UniGene Hs.406266; Hs.591588;
  • GeneID 3099
  • KEGG hsa:3099;
  • Human Hexokinase-2 Protein SEQUENCE
  • SEQUENCE 917 AA; 102380 MW; F17CE1938CF13880 CRC64;

    MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG

    LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN

    GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL

    GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD

    IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG

    DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM

    YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR

    KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN

    KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG

    KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS

    KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW

    GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF

    TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD

    VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE

    GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL

    GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV

    RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG

    LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG

    AALITAVACR IREAGQR

  • UCSC uc002snd.4; human.;


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