Human HK1 ELISA Kit

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  • Alternative name

    Human brain form hexokinase ELISA Kit;Human hexokinase type I ELISA Kit;Human HK I ELISA Kit;Human HK ELISA Kit;Human HK1-ta ELISA Kit;Human HK1-tb ELISA Kit;Human HK1-tc ELISA Kit;Human HKD ELISA Kit;Human HKI ELISA Kit;Human HMSNR ELISA Kit;Human HXK1 ELISA Kit;Human hexokinase ELISA Kit;Human hexokinase 1 ELISA Kit;Human hexokinase-1 ELISA Kit;Human glycolytic enzyme ELISA Kit;Human hexokinase I ELISA Kit;Human hexokinase IR ELISA Kit;

  • Catalog
    E018546
  • species
    Human
  • GeneHK1
  • Standard CurveHuman HK1 ELISA Kit
  • Other Species Mouse HK1 ELISA KitBovine HK1 ELISA KitRat Hk1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hexokinase 1 (HK1). No significant cross-reactivity or interference between Hexokinase 1 (HK1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 24.5pg/mL.
  • Intended UseHuman HK1 ELISA Kit allows for the in vitro quantitative determination of HK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexokinase 1 (HK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hexokinase 1 (HK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hexokinase 1 (HK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hexokinase 1 (HK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Hexokinase-1 Protein information
  • Uniprot ID HXK1_HUMAN
  • Uniprot AC Q9NNZ5;
  • UniGene Hs.370365;
  • GeneID 3098
  • KEGG hsa:3098;
  • Human Hexokinase-1 Protein SEQUENCE
  • SEQUENCE 917 AA; 102486 MW; F29A6837531C0594 CRC64;

    MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG

    LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE

    KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV

    GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD

    ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG

    DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM

    YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH

    NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN

    KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG

    KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR

    KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK

    RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF

    TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD

    VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ

    GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL

    GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV

    RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG

    LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG

    AALITAVGVR LRTEASS

  • UCSC uc001jpg.5; human. [P19367-1];



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