-
Alternative name
Hephaestin ELISA KIT; HEPH ELISA KIT; KIAA0698 ELISA KIT; UNQ2562/PRO6242 ELISA KIT
-
Catalog
E018431
-
species
Human
-
GeneHEPH
-
Other Species
Mouse HEPH ELISA Kit
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HEPH. No significant cross-reactivity or interference between human HEPH and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityThe minimum detectable dose of human HEPH is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
-
Intended UseHuman HEPH ELISA Kit allows for the in vitro quantitative determination of HEPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HEPH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HEPH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HEPH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HEPH bound in the initial step. The color development is stopped and the intensity of the color is measured.
-
Related products with Human HEPH ELISA Kit