Human HAMP ELISA Kit

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  • Alternative name

    Human liver-expressed antimicrobial peptide 1 ELISA Kit;Human LEAP-1 ELISA Kit;Human putative liver tumor regressor ELISA Kit;Human PLTR ELISA Kit;Human Hepc25 ELISA Kit;Human Hepc20 ELISA Kit;Human HEPC ELISA Kit;Human LEAP1 ELISA Kit;Human HFE2B ELISA Kit;Human hepcidin antimicrobial peptide ELISA Kit;Human hepcidin ELISA Kit;

  • Catalog
    E018423
  • species
    Human
  • GeneHAMP
  • Standard CurveHuman HAMP ELISA Kit
  • Other Species Mouse Hamp ELISA KitCanine HAMP ELISA KitPorcine HAMP ELISA KitRat Hamp ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HAMP. No significant cross-reactivity or interference between HAMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HAMP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL
  • Intended UseHuman HAMP ELISA Kit allows for the in vitro quantitative determination of HAMP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This HAMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HAMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: HAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-HAMP antibody and an HAMP-HRP conjugate. The assay sample and buffer are incubated together with HAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HAMP concentration since HAMP from samples and HAMP-HRP conjugate compete for the anti-HAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by HAMP from the sample, fewer sites are left to bind HAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HAMP concentration in each sample is interpolated from this standard curve.
  • Human Hepcidin-20 Protein information
  • Uniprot ID HEPC_HUMAN
  • Uniprot AC P81172; Q1HE14; Q9BY68;
  • UniGene Hs.8821;
  • GeneID 57817
  • KEGG hsa:57817;
  • Human Hepcidin-20 Protein SEQUENCE
  • SEQUENCE 84 AA; 9408 MW; 5F8DCA23D19D29F7 CRC64;

    MALSSQIWAA CLLLLLLLAS LTSGSVFPQQ TGQLAELQPQ DRAGARASWM

    PMFQRRRRRD THFPICIFCC GCCHRSKCGM CCKT

  • UCSC uc002nyw.4; human.;



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