SpecificityThis assay has high sensitivity and excellent specificity for detection of HAMP. No significant cross-reactivity or interference between HAMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HAMP and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman HAMP ELISA Kit allows for the in vitro quantitative determination of HAMP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description specificalIntended Uses: This HAMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HAMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: HAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-HAMP antibody and an HAMP-HRP conjugate. The assay sample and buffer are incubated together with HAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HAMP concentration since HAMP from samples and HAMP-HRP conjugate compete for the anti-HAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by HAMP from the sample, fewer sites are left to bind HAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HAMP concentration in each sample is interpolated from this standard curve.