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Human HNF1A ELISA Kit

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  • Alternative name

    Human HNF-1-alpha ELISA Kit;Human HNF-1A ELISA Kit;Human liver-specific transcription factor LF-B1 ELISA Kit;Human LFB1 ELISA Kit;Human Transcription factor 1 ELISA Kit;Human TCF-1 ELISA Kit;Human TCF1 ELISA Kit;Human HNF1 ELISA Kit;Human IDDM20 ELISA Kit;Human MODY3 ELISA Kit;Human HNF1 homeobox A ELISA Kit;Human hepatocyte nuclear factor 1-alpha ELISA Kit;Human albumin proximal factor ELISA Kit;Human hepatic nuclear factor 1 ELISA Kit;Human interferon production regulator factor ELISA Kit;Human transcription factor 1, hepatic ELISA Kit;
  • Catalog
    E018397
  • species
    Human
  • GeneHNF1A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Alpha (HNF1a). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Alpha (HNF1a) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman HNF1A ELISA Kit allows for the in vitro quantitative determination of HNF1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Alpha (HNF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Hepatocyte nuclear factor 1-alpha Protein information
  • Uniprot ID HNF1A_HUMAN
  • Uniprot AC Q2M3H2; Q99861;
  • UniGene Hs.654455;
  • GeneID 6927
  • KEGG hsa:6927;
  • Human Hepatocyte nuclear factor 1-alpha Protein SEQUENCE

  • SEQUENCE 631 AA; 67356 MW; 8327CD4FDC39254A CRC64;

    MVSKLSQLQT ELLAALLESG LSKEALIQAL GEPGPYLLAG EGPLDKGESC

    GGGRGELAEL PNGLGETRGS EDETDDDGED FTPPILKELE NLSPEEAAHQ

    KAVVETLLQE DPWRVAKMVK SYLQQHNIPQ REVVDTTGLN QSHLSQHLNK

    GTPMKTQKRA ALYTWYVRKQ REVAQQFTHA GQGGLIEEPT GDELPTKKGR

    RNRFKWGPAS QQILFQAYER QKNPSKEERE TLVEECNRAE CIQRGVSPSQ

    AQGLGSNLVT EVRVYNWFAN RRKEEAFRHK LAMDTYSGPP PGPGPGPALP

    AHSSPGLPPP ALSPSKVHGV RYGQPATSET AEVPSSSGGP LVTVSTPLHQ

    VSPTGLEPSH SLLSTEAKLV SAAGGPLPPV STLTALHSLE QTSPGLNQQP

    QNLIMASLPG VMTIGPGEPA SLGPTFTNTG ASTLVIGLAS TQAQSVPVIN

    SMGSSLTTLQ PVQFSQPLHP SYQQPLMPPV QSHVTQSPFM ATMAQLQSPH

    ALYSHKPEVA QYTHTGLLPQ TMLITDTTNL SALASLTPTK QVFTSDTEAS

    SESGLHTPAS QATTLHVPSQ DPAGIQHLQP AHRLSASPTV SSSSLVLYQS

    SDSSNGQSHL LPSNHSVIET FISTQMASSS Q

  • UCSC uc021rfb.2; human. [P20823-1];



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