Human HGFAC ELISA Kit

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  • Alternative name

    Human HGF activator ELISA Kit;Human HGFA ELISA Kit;Human hepatocyte growth factor activator ELISA Kit;

  • Catalog
    E018385
  • species
    Human
  • GeneHGFAC
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor Activator (HGFAC). No significant cross-reactivity or interference between Hepatocyte Growth Factor Activator (HGFAC) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.7pg/mL.
  • Intended UseHuman HGFAC ELISA Kit allows for the in vitro quantitative determination of HGFAC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor Activator (HGFAC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Growth Factor Activator (HGFAC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Growth Factor Activator (HGFAC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Growth Factor Activator (HGFAC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Hepatocyte growth factor activator long chain Protein information
  • Uniprot ID HGFA_HUMAN
  • Uniprot AC TSEGSAHR KWCATTHNYD RDRAWGYCVE ATPPPGGPAA LDPCASGPCL NGGSCSNTQD
  • UniGene Hs.104;
  • GeneID 3083
  • KEGG hsa:3083;
  • Human Hepatocyte growth factor activator long chain Protein SEQUENCE
  • SEQUENCE 655 AA; 70682 MW; 2CF72F1E1B862ED7 CRC64;

    MGRWAWVPSP WPPPGLGPFL LLLLLLLLLP RGFQPQPGGN RTESPEPNAT

    ATPAIPTILV TSVTSETPAT SAPEAEGPQS GGLPPPPRAV PSSSSPQAQA

    LTEDGRPCRF PFRYGGRMLH ACTSEGSAHR KWCATTHNYD RDRAWGYCVE

    ATPPPGGPAA LDPCASGPCL NGGSCSNTQD PQSYHCSCPR AFTGKDCGTE

    KCFDETRYEY LEGGDRWARV RQGHVEQCEC FGGRTWCEGT RHTACLSSPC

    LNGGTCHLIV ATGTTVCACP PGFAGRLCNI EPDERCFLGN GTGYRGVAST

    SASGLSCLAW NSDLLYQELH VDSVGAAALL GLGPHAYCRN PDNDERPWCY

    VVKDSALSWE YCRLEACESL TRVQLSPDLL ATLPEPASPG RQACGRRHKK

    RTFLRPRIIG GSSSLPGSHP WLAAIYIGDS FCAGSLVHTC WVVSAAHCFS

    HSPPRDSVSV VLGQHFFNRT TDVTQTFGIE KYIPYTLYSV FNPSDHDLVL

    IRLKKKGDRC ATRSQFVQPI CLPEPGSTFP AGHKCQIAGW GHLDENVSGY

    SSSLREALVP LVADHKCSSP EVYGADISPN MLCAGYFDCK SDACQGDSGG

    PLACEKNGVA YLYGIISWGD GCGRLHKPGV YTRVANYVDW INDRIRPPRR

    LVAPS

  • UCSC uc003ghc.4; human.;



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