Human HGF ELISA Kit

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  • Alternative name

    Human hepatopoietin-A ELISA Kit;Human Scatter factor ELISA Kit;Human SF ELISA Kit;Human HPTA ELISA Kit;Human DFNB39 ELISA Kit;Human F-TCF ELISA Kit;Human HGFB ELISA Kit;Human hepatocyte growth factor ELISA Kit;Human fibroblast-derived tumor cytotoxic factor ELISA Kit;Human hepatocyte growth factor (hepapoietin A; scatter factor) ELISA Kit;Human lung fibroblast-derived mitogen ELISA Kit;

  • Catalog
    E018381
  • species
    Human
  • GeneHGF
  • Other Species Mouse Hgf ELISA KitBovine HGF ELISA KitCanine HGF ELISA KitRat Hgf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human HGF. No significant cross-reactivity or interference between Human HGF and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.56 pg/ml
  • Intended UseHuman HGF ELISA Kit allows for the in vitro quantitative determination of HGF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Hepatocyte growth factor beta chain Protein information
  • Uniprot ID HGF_HUMAN
  • Uniprot AC Q9BYM0; Q9UDU6;
  • UniGene Hs.396530; Hs.561679;
  • GeneID 3082
  • KEGG hsa:3082;
  • Human Hepatocyte growth factor beta chain Protein SEQUENCE
  • SEQUENCE 728 AA; 83134 MW; 2D997938295ADD2F CRC64;

    MWVTKLLPAL LLQHVLLHLL LLPIAIPYAE GQRKRRNTIH EFKKSAKTTL

    IKIDPALKIK TKKVNTADQC ANRCTRNKGL PFTCKAFVFD KARKQCLWFP

    FNSMSSGVKK EFGHEFDLYE NKDYIRNCII GKGRSYKGTV SITKSGIKCQ

    PWSSMIPHEH SFLPSSYRGK DLQENYCRNP RGEEGGPWCF TSNPEVRYEV

    CDIPQCSEVE CMTCNGESYR GLMDHTESGK ICQRWDHQTP HRHKFLPERY

    PDKGFDDNYC RNPDGQPRPW CYTLDPHTRW EYCAIKTCAD NTMNDTDVPL

    ETTECIQGQG EGYRGTVNTI WNGIPCQRWD SQYPHEHDMT PENFKCKDLR

    ENYCRNPDGS ESPWCFTTDP NIRVGYCSQI PNCDMSHGQD CYRGNGKNYM

    GNLSQTRSGL TCSMWDKNME DLHRHIFWEP DASKLNENYC RNPDDDAHGP

    WCYTGNPLIP WDYCPISRCE GDTTPTIVNL DHPVISCAKT KQLRVVNGIP

    TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI

    HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP

    NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV

    TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP

    NRPGIFVRVA YYAKWIHKII LTYKVPQS

  • UCSC uc003uhl.4; human. [P14210-1];



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