Human CD13 ELISA Kit

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  • Alternative name

    Aminopeptidase N ELISA KIT; Alanyl aminopeptidase ELISA KIT; Aminopeptidase M ELISA KIT; AP-M ELISA KIT; Microsomal aminopeptidase ELISA KIT; Myeloid plasma membrane glycoprotein CD13 ELISA KIT; gp150 ELISA KIT; CD_antigen: CD13ANPEP ELISA KIT; APN ELISA KIT; CD13 ELISA KIT; PEPN ELISA KIT; AP-N ELISA KIT; hAPN ELISA KIT; AP-M ELISA KIT

  • Catalog
  • species
  • GeneCD13
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.938ng/ml
  • Intended UseHuman CD13 ELISA Kit allows for the in vitro quantitative determination of CD13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CD13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CD13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD13 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD13 can be calculated.

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