Human HCII ELISA Kit

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  • Alternative name

    Heparin cofactor 2 ELISA KIT; Heparin cofactor II ELISA KIT; HC-II ELISA KIT; Protease inhibitor leuserpin-2 ELISA KIT; HLS2 ELISA KIT; Serpin D1SERPIND1 ELISA KIT; HCF2 ELISA KIT; HC-II ELISA KIT; HLS2 ELISA KIT

  • Catalog
    E018296
  • species
    Human
  • GeneHCII
  • Standard CurveHuman HCII ELISA Kit
  • Other Species Human HCII-T ELISA KitMouse HCII ELISA KitMouse HCII-T ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Heparin Cofactor II (HCII). No significant cross-reactivity or interference between Heparin Cofactor II (HCII) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.71ng/mL.
  • Intended UseHuman HCII ELISA Kit allows for the in vitro quantitative determination of HCII , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Cofactor II (HCII). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparin Cofactor II (HCII). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparin Cofactor II (HCII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparin Cofactor II (HCII) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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