Human HS6ST2 ELISA Kit

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  • Alternative name

    Human HS6ST-2 ELISA Kit;Human heparan sulfate 6-O-sulfotransferase 2 ELISA Kit;Human heparan-sulfate 6-O-sulfotransferase 2 ELISA Kit;

  • Catalog
    E018266
  • species
    Human
  • GeneHS6ST2
  • Standard CurveHuman HS6ST2 ELISA Kit
  • Other Species Mouse Hs6st2 ELISA KitChicken HS6ST2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2). No significant cross-reactivity or interference between Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman HS6ST2 ELISA Kit allows for the in vitro quantitative determination of HS6ST2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Heparan-sulfate 6-O-sulfotransferase 2 Protein information
  • Uniprot ID H6ST2_HUMAN
  • Uniprot AC Q8N3T4; Q8NBN4; Q96SJ4;
  • UniGene Hs.385956;
  • GeneID 90161
  • KEGG hsa:90161;
  • Human Heparan-sulfate 6-O-sulfotransferase 2 Protein SEQUENCE
  • SEQUENCE 605 AA; 69130 MW; 10528AC424935B13 CRC64;

    MALPACAVRE FEPPRQPERG APVRTTCPRR HSRVEAELAA SRPGSVAASV

    RAGPPRGVSH GFHTRPLLDK PRKASSSLAG AACAPLFALL SRGRRRRMHV

    LRRRWDLGSL CRALLTRGLA ALGHSLKHVL GAIFSKIFGP MASVGNMDEK

    SNKLLLALVM LFLFAVIVLQ YVCPGTECQL LRLQAFSSPV PDPYRSEDES

    SARFVPRYNF TRGDLLRKVD FDIKGDDLIV FLHIQKTGGT TFGRHLVRNI

    QLEQPCECRV GQKKCTCHRP GKRETWLFSR FSTGWSCGLH ADWTELTSCV

    PSVVDGKRDA RLRPSRNFHY ITILRDPVSR YLSEWRHVQR GATWKASLHV

    CDGRPPTSEE LPSCYTGDDW SGCPLKEFMD CPYNLANNRQ VRMLSDLTLV

    GCYNLSVMPE KQRNKVLLES AKSNLKHMAF FGLTEFQRKT QYLFEKTFNM

    NFISPFTQYN TTRASSVEIN EEIQKRIEGL NFLDMELYSY AKDLFLQRYQ

    FMRQKEHQEA RRKRQEQRKF LKGRLLQTHF QSQGQGQSQN PNQNQSQNPN

    PNANQNLTQN LMQNLTQSLS QKENRESPKQ NSGKEQNDNT SNGTNDYIGS

    VEKWR

  • UCSC uc011mvd.2; human. [Q96MM7-1];



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