Human HCK ELISA Kit

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  • Alternative name

    Human hematopoietic cell kinase ELISA Kit;Human hemopoietic cell kinase ELISA Kit;Human p59-HCK/p60-HCK ELISA Kit;Human p59Hck ELISA Kit;Human p61Hck ELISA Kit;Human JTK9 ELISA Kit;Human HCK proto-oncogene, Src family tyrosine kinase ELISA Kit;Human tyrosine-protein kinase HCK ELISA Kit;

  • Catalog
    E018234
  • species
    Human
  • GeneHCK
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human HCK. No significant cross-reactivity or interference between human HCK and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human HCK is typically less than 4.69 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman HCK ELISA Kit allows for the in vitro quantitative determination of HCK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HCK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HCK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HCK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCK bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Tyrosine-protein kinase HCK Protein information
  • Uniprot ID HCK_HUMAN
  • Uniprot AC Q5T7K2; Q96CC0; Q9H5Y5; Q9NUA4; Q9UMJ5;
  • UniGene Hs.655210;
  • GeneID 3055
  • KEGG hsa:3055;
  • Human Tyrosine-protein kinase HCK Protein SEQUENCE
  • SEQUENCE 526 AA; 59600 MW; 847E877A0A641725 CRC64;

    MGGRSSCEDP GCPRDEERAP RMGCMKSKFL QVGGNTFSKT ETSASPHCPV

    YVPDPTSTIK PGPNSHNSNT PGIREAGSED IIVVALYDYE AIHHEDLSFQ

    KGDQMVVLEE SGEWWKARSL ATRKEGYIPS NYVARVDSLE TEEWFFKGIS

    RKDAERQLLA PGNMLGSFMI RDSETTKGSY SLSVRDYDPR QGDTVKHYKI

    RTLDNGGFYI SPRSTFSTLQ ELVDHYKKGN DGLCQKLSVP CMSSKPQKPW

    EKDAWEIPRE SLKLEKKLGA GQFGEVWMAT YNKHTKVAVK TMKPGSMSVE

    AFLAEANVMK TLQHDKLVKL HAVVTKEPIY IITEFMAKGS LLDFLKSDEG

    SKQPLPKLID FSAQIAEGMA FIEQRNYIHR DLRAANILVS ASLVCKIADF

    GLARVIEDNE YTAREGAKFP IKWTAPEAIN FGSFTIKSDV WSFGILLMEI

    VTYGRIPYPG MSNPEVIRAL ERGYRMPRPE NCPEELYNIM MRCWKNRPEE

    RPTFEYIQSV LDDFYTATES QYQQQP

  • UCSC uc002wxi.4; human. [P08631-1];



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