Human HPX ELISA Kit

Alternative name
Human beta-1B-glycoprotein ELISA Kit;Human HX ELISA Kit;Human hemopexin ELISA Kit;
Catalog
E018228
species
Human
GeneHPX
SpecificityThis assay has high sensitivity and excellent specificity for detection of HPX. No significant cross-reactivity or interference between HPX and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HPX and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0pg/mL.
Intended UseHuman HPX ELISA Kit allows for the in vitro quantitative determination of HPX , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This HPX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HPX. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||HPX ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HPX. Standards or samples are then added to the microtiter plate wells and HPX if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HPX present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HPX are added to each well to "sandwich" the HPX immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HPX and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPX concentration in each sample is interpolated from this standard curve.
Human Hemopexin Protein information
Uniprot ID HEMO_HUMAN
Uniprot AC P02790; B2R957;
UniGene Hs.426485;
GeneID 3263
KEGG hsa:3263;
Human Hemopexin Protein SEQUENCE
SEQUENCE 462 AA; 51676 MW; 054B44D0603763B8 CRC64;
MARVLGAPVA LGLWSLCWSL AIATPLPPTS AHGNVAEGET KPDPDVTERC
SDGWSFDATT LDDNGTMLFF KGEFVWKSHK WDRELISERW KNFPSPVDAA
FRQGHNSVFL IKGDKVWVYP PEKKEKGYPK LLQDEFPGIP SPLDAAVECH
RGECQAEGVL FFQGDREWFW DLATGTMKER SWPAVGNCSS ALRWLGRYYC
FQGNQFLRFD PVRGEVPPRY PRDVRDYFMP CPGRGHGHRN GTGHGNSTHH
GPEYMRCSPH LVLSALTSDN HGATYAFSGT HYWRLDTSRD GWHSWPIAHQ
WPQGPSAVDA AFSWEEKLYL VQGTQVYVFL TKGGYTLVSG YPKRLEKEVG
TPHGIILDSV DAAFICPGSS RLHIMAGRRL WWLDLKSGAQ ATWTELPWPH
EKVDGALCME KSLGPNSCSA NGPGLYLIHG PNLYCYSDVE KLNAAKALPQ
PQNVTSLLGC TH
UCSC uc001mdg.3; human.;

Category

Human HPX ELISA Kit

Human beta-1B-glycoprotein ELISA Kit;Human HX ELISA Kit;Human hemopexin ELISA Kit;