Intended UseHuman HJV ELISA Kit allows for the in vitro quantitative determination of HJV , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of hemojuvelin in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to hemojuvelin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled hemojuvelin and unlabeled hemojuvelin (Standards or samples) with the pre-coated antibody specific to hemojuvelin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of hemojuvelin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of hemojuvelin in the sample.