Human AADAT ELISA Kit

Alternative name
Human KAT/AadAT ELISA Kit;Human 2-aminoadipate aminotransferase ELISA Kit;Human 2-aminoadipate transaminase ELISA Kit;Human alpha-aminoadipate aminotransferase ELISA Kit;Human AadAT ELISA Kit;Human kynurenine aminotransferase II ELISA Kit;Human kynurenine--oxoglutarate aminotransferase II ELISA Kit;Human kynurenine--oxoglutarate transaminase 2 ELISA Kit;Human kynurenine--oxoglutarate transaminase II ELISA Kit;Human KAT2 ELISA Kit;Human KATII ELISA Kit;Human KYAT2 ELISA Kit;Human aminoadipate aminotransferase ELISA Kit;Human kynurenine/alpha-aminoadipate aminotransferase, mitochondrial ELISA Kit;Human L kynurenine/alpha aminoadipate aminotransferase ELISA Kit;
Catalog
E001821
species
Human
GeneAADAT
Standard Curve
Other Species Mouse Aadat ELISA Kit Bovine AADAT ELISA Kit Rat Aadat ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Aminoadipate Aminotransferase (AADAT). No significant cross-reactivity or interference between Aminoadipate Aminotransferase (AADAT) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.067ng/mL.
Intended UseHuman AADAT ELISA Kit allows for the in vitro quantitative determination of AADAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoadipate Aminotransferase (AADAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoadipate Aminotransferase (AADAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoadipate Aminotransferase (AADAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aminoadipate Aminotransferase (AADAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial Protein information
Uniprot ID AADAT_HUMAN
Uniprot AC Q8N5Z0; B3KP84; Q9UL02;
UniGene Hs.529735;
GeneID 51166
KEGG hsa:51166;
Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial Protein SEQUENCE
SEQUENCE 425 AA; 47352 MW; 448CCAAB2173A7BA CRC64;
MNYARFITAA SAARNPSPIR TMTDILSRGP KSMISLAGGL PNPNMFPFKT
AVITVENGKT IQFGEEMMKR ALQYSPSAGI PELLSWLKQL QIKLHNPPTI
HYPPSQGQMD LCVTSGSQQG LCKVFEMIIN PGDNVLLDEP AYSGTLQSLH
PLGCNIINVA SDESGIVPDS LRDILSRWKP EDAKNPQKNT PKFLYTVPNG
NNPTGNSLTS ERKKEIYELA RKYDFLIIED DPYYFLQFNK FRVPTFLSMD
VDGRVIRADS FSKIISSGLR IGFLTGPKPL IERVILHIQV STLHPSTFNQ
LMISQLLHEW GEEGFMAHVD RVIDFYSNQK DAILAAADKW LTGLAEWHVP
AAGMFLWIKV KGINDVKELI EEKAVKMGVL MLPGNAFYVD SSAPSPYLRA
SFSSASPEQM DVAFQVLAQL IKESL
UCSC uc003isr.4; human. [Q8N5Z0-1];

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