Intended UseHuman HBE1 ELISA Kit allows for the in vitro quantitative determination of HBE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for HBE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated HBE1. A competitive inhibition reaction is launched between HBE1 (Standards or samples) and biotin-conjugated HBE1 with the pre-coated HBE1 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of HBE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Hemoglobin subunit epsilon Protein information