SpecificityThis assay has high sensitivity and excellent specificity for detection of Hemoglobin Gamma 1 (HBg1).
No significant cross-reactivity or interference between Hemoglobin Gamma 1 (HBg1) and analogues was observed.
Intended UseHuman HBG1 ELISA Kit allows for the in vitro quantitative determination of HBG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin Gamma 1 (HBg1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin Gamma 1 (HBg1) and unlabeled Hemoglobin Gamma 1 (HBg1) (Standards or samples) with the pre-coated antibody specific to Hemoglobin Gamma 1 (HBg1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin Gamma 1 (HBg1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin Gamma 1 (HBg1) in the sample.
Human Hemoglobin subunit gamma-1 Protein information
Uniprot ID
HBG1_HUMAN
Uniprot AC
P69891; P02096; P62027; Q549G1; Q8TDA1; Q96FH7;