Human ACY1 ELISA Kit

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  • Alternative name

    Human ACY-1 ELISA Kit;Human N-acyl-L-amino-acid amidohydrolase ELISA Kit;Human ACY1D ELISA Kit;Human HEL-S-5 ELISA Kit;Human aminoacylase 1 ELISA Kit;Human aminoacylase-1 ELISA Kit;Human acylase ELISA Kit;Human epididymis secretory protein Li 5 ELISA Kit;

  • Catalog
    E001815
  • species
    Human
  • GeneACY1
  • Standard CurveHuman ACY1 ELISA Kit
  • Other Species Mouse Acy1 ELISA KitPorcine ACY1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Aminoacylase 1 (ACY1). No significant cross-reactivity or interference between Aminoacylase 1 (ACY1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman ACY1 ELISA Kit allows for the in vitro quantitative determination of ACY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoacylase 1 (ACY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoacylase 1 (ACY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoacylase 1 (ACY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aminoacylase 1 (ACY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Aminoacylase-1 Protein information
  • Uniprot ID ACY1_HUMAN
  • Uniprot AC Q03154; C9J6I6; C9J9D8; C9JWD4;
  • UniGene Hs.334707;
  • GeneID 95
  • KEGG hsa:95;
  • Human Aminoacylase-1 Protein SEQUENCE
  • SEQUENCE 408 AA; 45885 MW; 293350CD7759826C CRC64;

    MTSKGPEEEH PSVTLFRQYL RIRTVQPKPD YGAAVAFFEE TARQLGLGCQ

    KVEVAPGYVV TVLTWPGTNP TLSSILLNSH TDVVPVFKEH WSHDPFEAFK

    DSEGYIYARG AQDMKCVSIQ YLEAVRRLKV EGHRFPRTIH MTFVPDEEVG

    GHQGMELFVQ RPEFHALRAG FALDEGIANP TDAFTVFYSE RSPWWVRVTS

    TGRPGHASRF MEDTAAEKLH KVVNSILAFR EKEWQRLQSN PHLKEGSVTS

    VNLTKLEGGV AYNVIPATMS ASFDFRVAPD VDFKAFEEQL QSWCQAAGEG

    VTLEFAQKWM HPQVTPTDDS NPWWAAFSRV CKDMNLTLEP EIMPAATDNR

    YIRAVGVPAL GFSPMNRTPV LLHDHDERLH EAVFLRGVDI YTRLLPALAS

    VPALPSDS

  • UCSC uc021wzb.2; human. [Q03154-1];


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