Intended UseHuman HP IgM ELISA Kit allows for the in vitro quantitative determination of HP IgM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: Anti-HP-IgM ELISA kit applies the competitive enzyme immunoassay technique utilizing a HP-IgM antigen and an anti-HP-IgM-HRP conjugate. The assay sample and buffer are incubated together with anti-HP-IgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-HP-IgM concentration since anti-HP-IgM from samples and anti-HP-IgM-HRP conjugate compete for the HP-IgM antigen binding site. Since the number of sites is limited, as more sites are occupied by anti-HP-IgM from the sample, fewer sites are left to bind anti-HP-IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-HP-IgM concentration in each sample is interpolated from this standard curve.