Intended UseHuman Hp-IgM ELISA Kit allows for the in vitro quantitative determination of Hp-IgM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: For the qualitative detection of human Helicobacter pylori IgM (Hp-IgM) concentration in serum, plasma and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with Hp. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Hp-IgM and incubated. Then substrate solutions are added to each well. Only those wells that contain Hp-IgM and enzymeconjugated Hp-IgM will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.